Cargando…

Construction of a Stability Landscape of the CH3 Domain of Human IgG1 by Combining Directed Evolution with High Throughput Sequencing

One of the most important but still poorly understood issues in protein chemistry is the relationship between sequence and stability of proteins. Here, we present a method for analyzing the influence of each individual residue on the foldability and stability of an entire protein. A randomly mutated...

Descripción completa

Detalles Bibliográficos
Autores principales:	Traxlmayr, Michael W., Hasenhindl, Christoph, Hackl, Matthias, Stadlmayr, Gerhard, Rybka, Jakub D., Borth, Nicole, Grillari, Johannes, Rüker, Florian, Obinger, Christian
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2012
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469823/ https://www.ncbi.nlm.nih.gov/pubmed/22846908 http://dx.doi.org/10.1016/j.jmb.2012.07.017

Ejemplares similares

Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution
por: Traxlmayr, Michael W, et al.
Publicado: (2014)

Stability assessment on a library scale: a rapid method for the evaluation of the commutability and insertion of residues in C-terminal loops of the CH3 domains of IgG1-Fc
por: Hasenhindl, Christoph, et al.
Publicado: (2013)

Directed evolution of stabilized IgG1-Fc scaffolds by application of strong heat shock to libraries displayed on yeast
por: Traxlmayr, Michael W., et al.
Publicado: (2012)

Engineered IgG1‐Fc – one fragment to bind them all
por: Lobner, Elisabeth, et al.
Publicado: (2016)

Creating stable stem regions for loop elongation in Fcabs — Insights from combining yeast surface display, in silico loop reconstruction and molecular dynamics simulations
por: Hasenhindl, Christoph, et al.
Publicado: (2014)

Molecular Dynamics Simulation of the Crystallizable Fragment of IgG1—Insights for the Design of Fcabs
por: Lai, Balder, et al.
Publicado: (2014)

Bispecific T-Cell Engagers Targeting Membrane-Bound IgE
por: Rodak, Aleksandra, et al.
Publicado: (2021)

Stabilization of the CD81 Large Extracellular Loop with De Novo Disulfide Bonds Improves Its Amenability for Peptide Grafting
por: Vogt, Stefan, et al.
Publicado: (2018)

An engineered CD81‐based combinatorial library for selecting recombinant binders to cell surface proteins: Laminin binding CD81 enhances cellular uptake of extracellular vesicles
por: Vogt, Stefan, et al.
Publicado: (2021)

Bispecific antibodies with Fab-arms featuring exchanged antigen-binding constant domains
por: Benedetti, Filippo, et al.
Publicado: (2021)

Stabilisation of the Fc Fragment of Human IgG1 by Engineered Intradomain Disulfide Bonds
por: Wozniak-Knopp, Gordana, et al.
Publicado: (2012)

Interactions of IgG1 CH2 and CH3 Domains with FcRn
por: Ying, Tianlei, et al.
Publicado: (2014)

An antibody with Fab-constant domains exchanged for a pair of C(H)3 domains
por: Wozniak-Knopp, Gordana, et al.
Publicado: (2018)

A Tetravalent Biparatopic Antibody Causes Strong HER2 Internalization and Inhibits Cellular Proliferation
por: Benedetti, Filippo, et al.
Publicado: (2021)

Bispecific mAb(2) Antibodies Targeting CD59 Enhance the Complement-Dependent Cytotoxicity Mediated by Rituximab
por: Stadlbauer, Katharina, et al.
Publicado: (2022)

The differential ability of human IgG1 and IgG4 to activate complement is determined by the COOH-terminal sequence of the CH2 domain
Publicado: (1991)

Stabilization of soluble high‐affinity T‐cell receptor with de novo disulfide bonds
por: Sádio, Flávio, et al.
Publicado: (2019)

An engineered human IgG1 CH2 domain with decreased aggregation and nonspecific binding
por: Cao, Guangcan, et al.
Publicado: (2019)

Endogenous microRNA clusters outperform chimeric sequence clusters in Chinese hamster ovary cells
por: Klanert, Gerald, et al.
Publicado: (2014)

Mutation of Y407 in the CH3 domain dramatically alters glycosylation and structure of human IgG
por: Rose, Rebecca J., et al.
Publicado: (2013)

Insights into the IgG heavy chain engineering patent landscape as applied to IgG4 antibody development
por: Dumet, Christophe, et al.
Publicado: (2019)

Turning the tables on cytomegalovirus: targeting viral Fc receptors by CARs containing mutated CH2–CH3 IgG spacer domains
por: Proff, Julia, et al.
Publicado: (2018)

High Throughput Isolation and Glycosylation Analysis of IgG–Variability and Heritability of the IgG Glycome in Three Isolated Human Populations
por: Pučić, Maja, et al.
Publicado: (2011)

Analysis of microRNA transcription and post-transcriptional processing by Dicer in the context of CHO cell proliferation
por: Hackl, Matthias, et al.
Publicado: (2014)

Serum IgG4 levels outperform IgG4/IgG RNA ratio in differential diagnosis of IgG4-related disease
por: Schulte, Lucas A., et al.
Publicado: (2020)

Subclasses of allergen-specific IgG: Serum IgG2 and IgG3 levels are not predicted by IgG1/IgG4 levels
por: MacGlashan, Donald, et al.
Publicado: (2021)

Stable overexpression of miR-17 enhances recombinant protein production of CHO cells()
por: Jadhav, Vaibhav, et al.
Publicado: (2014)

Genetic Removal of the CH1 Exon Enables the Production of Heavy Chain-Only IgG in Mice
por: Zhang, Tianyi, et al.
Publicado: (2018)

CH2 Domain of Mouse IgG3 Governs Antibody Oligomerization, Increases Functional Affinity to Multivalent Antigens and Enhances Hemagglutination
por: Klaus, Tomasz, et al.
Publicado: (2018)

The antiinflammatory activity of IgG: the intravenous IgG paradox
por: Nimmerjahn, Falk, et al.
Publicado: (2007)

Identification of Activating Mutations in the Transmembrane and Extracellular Domains of EGFR
por: Wagner, Anja, et al.
Publicado: (2022)

Enriched blood IgG sialylation attenuates IgG-mediated and IgG-controlled-IgE-mediated allergic reactions
por: Petry, Janina, et al.
Publicado: (2020)

Association of dengue infection with anti-alpha-gal antibodies, IgM, IgG, IgG1, and IgG2
por: Olajiga, Olayinka M., et al.
Publicado: (2022)

Corrigendum: Genetic Removal of the CH1 Exon Enables the Production of Heavy Chain-Only IgG in Mice
por: Zhang, Tianyi, et al.
Publicado: (2019)

Landscape of the RBD-specific IgG, IgM, and IgA responses triggered by the inactivated virus vaccine against the Omicron variant
por: Xue, Jun-biao, et al.
Publicado: (2022)

Specificity of fc receptors for IgG2a, IgG1/IgG2b, and IgE on rat macrophages
Publicado: (1981)

Annotation of additional evolutionary conserved microRNAs in CHO cells from updated genomic data
por: Diendorfer, Andreas B., et al.
Publicado: (2015)

The Expression of IgG and IgG4 in Orbital MALT Lymphoma: The Similarities and Differences of IgG4-Related Diseases
por: Li, Kai-Ming, et al.
Publicado: (2020)

High-Throughput IgG Epitope Mapping of Tetanus Neurotoxin: Implications for Immunotherapy and Vaccine Design
por: De-Simone, Salvatore G., et al.
Publicado: (2023)

Lymphocyte membrane IgG and secreted IgG are structurally and allotypically distinct
Publicado: (1980)

Cannot write session to /tmp/vufind_sessions/sess_i41vh91kn1283kflvrkffnanc3