Polymorphisms in the CYP2E1 and GSTM1 Genes as Possible Protection Factors for Leprosy Patients

BACKGROUND: The CYP2E1 and GSTM1 genes encode metabolic enzymes that have key functions in drug modification and elimination. METHODOLOGY/PRINCIPAL FINDINGS: We investigated the possible effects of CYP2E1 and GSTM1 polymorphisms in 71 leprosy patients and in 110 individuals from the general populati...

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Autores principales: Pinto, Pablo, Salgado, Claudio Guedes, Santos, Ney, Alencar, Dayse O., Santos, Sidney, Hutz, Mara H., Ribeiro-dos-Santos, Ândrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3471857/
https://www.ncbi.nlm.nih.gov/pubmed/23077626
http://dx.doi.org/10.1371/journal.pone.0047498
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author Pinto, Pablo
Salgado, Claudio Guedes
Santos, Ney
Alencar, Dayse O.
Santos, Sidney
Hutz, Mara H.
Ribeiro-dos-Santos, Ândrea
author_facet Pinto, Pablo
Salgado, Claudio Guedes
Santos, Ney
Alencar, Dayse O.
Santos, Sidney
Hutz, Mara H.
Ribeiro-dos-Santos, Ândrea
author_sort Pinto, Pablo
collection PubMed
description BACKGROUND: The CYP2E1 and GSTM1 genes encode metabolic enzymes that have key functions in drug modification and elimination. METHODOLOGY/PRINCIPAL FINDINGS: We investigated the possible effects of CYP2E1 and GSTM1 polymorphisms in 71 leprosy patients and in 110 individuals from the general population. The GSTM1*0 null allele and INDEL CYP2E1*1D mutant genotypes were analyzed by conventional PCR, while CYP2E1 SNPs (1053C>T, 1293G>C and 7632T>A) were determined by RT-PCR. In leprosy patients, the GSTM1*0 and CYP2E1*5 alleles and the combined alleles GSTM1*0/CYP2E1*6 and GSTM1*0/CYP2E1*5 were significantly related to a baciloscopic index (BI) (BI<3), while the CYP2E1*6 allele was related to a better clinical evolution in the leprosy spectrum. CONCLUSIONS/SIGNIFICANCE: Therefore, GSTM1*0, CYP2E1*5 and CYP2E1*6 may be possible protection factors for leprosy patients.
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spelling pubmed-34718572012-10-17 Polymorphisms in the CYP2E1 and GSTM1 Genes as Possible Protection Factors for Leprosy Patients Pinto, Pablo Salgado, Claudio Guedes Santos, Ney Alencar, Dayse O. Santos, Sidney Hutz, Mara H. Ribeiro-dos-Santos, Ândrea PLoS One Research Article BACKGROUND: The CYP2E1 and GSTM1 genes encode metabolic enzymes that have key functions in drug modification and elimination. METHODOLOGY/PRINCIPAL FINDINGS: We investigated the possible effects of CYP2E1 and GSTM1 polymorphisms in 71 leprosy patients and in 110 individuals from the general population. The GSTM1*0 null allele and INDEL CYP2E1*1D mutant genotypes were analyzed by conventional PCR, while CYP2E1 SNPs (1053C>T, 1293G>C and 7632T>A) were determined by RT-PCR. In leprosy patients, the GSTM1*0 and CYP2E1*5 alleles and the combined alleles GSTM1*0/CYP2E1*6 and GSTM1*0/CYP2E1*5 were significantly related to a baciloscopic index (BI) (BI<3), while the CYP2E1*6 allele was related to a better clinical evolution in the leprosy spectrum. CONCLUSIONS/SIGNIFICANCE: Therefore, GSTM1*0, CYP2E1*5 and CYP2E1*6 may be possible protection factors for leprosy patients. Public Library of Science 2012-10-15 /pmc/articles/PMC3471857/ /pubmed/23077626 http://dx.doi.org/10.1371/journal.pone.0047498 Text en © 2012 Pinto et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Pinto, Pablo
Salgado, Claudio Guedes
Santos, Ney
Alencar, Dayse O.
Santos, Sidney
Hutz, Mara H.
Ribeiro-dos-Santos, Ândrea
Polymorphisms in the CYP2E1 and GSTM1 Genes as Possible Protection Factors for Leprosy Patients
title Polymorphisms in the CYP2E1 and GSTM1 Genes as Possible Protection Factors for Leprosy Patients
title_full Polymorphisms in the CYP2E1 and GSTM1 Genes as Possible Protection Factors for Leprosy Patients
title_fullStr Polymorphisms in the CYP2E1 and GSTM1 Genes as Possible Protection Factors for Leprosy Patients
title_full_unstemmed Polymorphisms in the CYP2E1 and GSTM1 Genes as Possible Protection Factors for Leprosy Patients
title_short Polymorphisms in the CYP2E1 and GSTM1 Genes as Possible Protection Factors for Leprosy Patients
title_sort polymorphisms in the cyp2e1 and gstm1 genes as possible protection factors for leprosy patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3471857/
https://www.ncbi.nlm.nih.gov/pubmed/23077626
http://dx.doi.org/10.1371/journal.pone.0047498
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