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Housekeeping Gene Selection Advisory: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and β-Actin Are Targets of miR-644a

Results of overexpression or downregulation of a microRNA (miRNA) on its target mRNA expression are often validated by reverse-transcription and quantitative PCR analysis using an appropriate housekeeping gene as an internal control. The possible direct or indirect effects of a miRNA on the expressi...

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Detalles Bibliográficos
Autores principales: Sikand, Kavleen, Singh, Jagjit, Ebron, Jey Sabith, Shukla, Girish C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472982/
https://www.ncbi.nlm.nih.gov/pubmed/23091630
http://dx.doi.org/10.1371/journal.pone.0047510