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Retrograde tracing and toe spreading after experimental autologous nerve transplantation and crush injury of the sciatic nerve: a descriptive methodological study

Evaluation of functional and structural recovery after peripheral nerve injury is crucial to determine the therapeutic effect of a nerve repair strategy. In the present study, we examined the relationship between the structural evaluation of regeneration by means of retrograde tracing and the functi...

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Autores principales: van Neerven, Sabien GA, Bozkurt, Ahmet, O’Dey, Dan Mon, Scheffel, Juliane, Boecker, Arne H, Stromps, Jan-Philipp, Dunda, Sebastian, Brook, Gary A, Pallua, Norbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3473253/
https://www.ncbi.nlm.nih.gov/pubmed/22546145
http://dx.doi.org/10.1186/1749-7221-7-5
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author van Neerven, Sabien GA
Bozkurt, Ahmet
O’Dey, Dan Mon
Scheffel, Juliane
Boecker, Arne H
Stromps, Jan-Philipp
Dunda, Sebastian
Brook, Gary A
Pallua, Norbert
author_facet van Neerven, Sabien GA
Bozkurt, Ahmet
O’Dey, Dan Mon
Scheffel, Juliane
Boecker, Arne H
Stromps, Jan-Philipp
Dunda, Sebastian
Brook, Gary A
Pallua, Norbert
author_sort van Neerven, Sabien GA
collection PubMed
description Evaluation of functional and structural recovery after peripheral nerve injury is crucial to determine the therapeutic effect of a nerve repair strategy. In the present study, we examined the relationship between the structural evaluation of regeneration by means of retrograde tracing and the functional analysis of toe spreading. Two standardized rat sciatic nerve injury models were used to address this relationship. As such, animals received either a 2 cm sciatic nerve defect (neurotmesis) followed by autologous nerve transplantation (ANT animals) or a crush injury with spontaneous recovery (axonotmesis; CI animals). Functional recovery of toe spreading was observed over an observation period of 84 days. In contrast to CI animals, ANT animals did not reach pre-surgical levels of toe spreading. After the observation period, the lipophilic dye DiI was applied to label sensory and motor neurons in dorsal root ganglia (DRG; sensory neurons) and spinal cord (motor neurons), respectively. No statistical difference in motor or sensory neuron counts could be detected between ANT and CI animals. In the present study we could indicate that there was no direct relationship between functional recovery (toe spreading) measured by SSI and the number of labelled (motor and sensory) neurons evaluated by retrograde tracing. The present findings demonstrate that a multimodal approach with a variety of independent evaluation tools is essential to understand and estimate the therapeutic benefit of a nerve repair strategy.
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spelling pubmed-34732532012-10-23 Retrograde tracing and toe spreading after experimental autologous nerve transplantation and crush injury of the sciatic nerve: a descriptive methodological study van Neerven, Sabien GA Bozkurt, Ahmet O’Dey, Dan Mon Scheffel, Juliane Boecker, Arne H Stromps, Jan-Philipp Dunda, Sebastian Brook, Gary A Pallua, Norbert J Brachial Plex Peripher Nerve Inj Research Article Evaluation of functional and structural recovery after peripheral nerve injury is crucial to determine the therapeutic effect of a nerve repair strategy. In the present study, we examined the relationship between the structural evaluation of regeneration by means of retrograde tracing and the functional analysis of toe spreading. Two standardized rat sciatic nerve injury models were used to address this relationship. As such, animals received either a 2 cm sciatic nerve defect (neurotmesis) followed by autologous nerve transplantation (ANT animals) or a crush injury with spontaneous recovery (axonotmesis; CI animals). Functional recovery of toe spreading was observed over an observation period of 84 days. In contrast to CI animals, ANT animals did not reach pre-surgical levels of toe spreading. After the observation period, the lipophilic dye DiI was applied to label sensory and motor neurons in dorsal root ganglia (DRG; sensory neurons) and spinal cord (motor neurons), respectively. No statistical difference in motor or sensory neuron counts could be detected between ANT and CI animals. In the present study we could indicate that there was no direct relationship between functional recovery (toe spreading) measured by SSI and the number of labelled (motor and sensory) neurons evaluated by retrograde tracing. The present findings demonstrate that a multimodal approach with a variety of independent evaluation tools is essential to understand and estimate the therapeutic benefit of a nerve repair strategy. BioMed Central 2012-04-30 /pmc/articles/PMC3473253/ /pubmed/22546145 http://dx.doi.org/10.1186/1749-7221-7-5 Text en Copyright © 2012 van Neerven et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
van Neerven, Sabien GA
Bozkurt, Ahmet
O’Dey, Dan Mon
Scheffel, Juliane
Boecker, Arne H
Stromps, Jan-Philipp
Dunda, Sebastian
Brook, Gary A
Pallua, Norbert
Retrograde tracing and toe spreading after experimental autologous nerve transplantation and crush injury of the sciatic nerve: a descriptive methodological study
title Retrograde tracing and toe spreading after experimental autologous nerve transplantation and crush injury of the sciatic nerve: a descriptive methodological study
title_full Retrograde tracing and toe spreading after experimental autologous nerve transplantation and crush injury of the sciatic nerve: a descriptive methodological study
title_fullStr Retrograde tracing and toe spreading after experimental autologous nerve transplantation and crush injury of the sciatic nerve: a descriptive methodological study
title_full_unstemmed Retrograde tracing and toe spreading after experimental autologous nerve transplantation and crush injury of the sciatic nerve: a descriptive methodological study
title_short Retrograde tracing and toe spreading after experimental autologous nerve transplantation and crush injury of the sciatic nerve: a descriptive methodological study
title_sort retrograde tracing and toe spreading after experimental autologous nerve transplantation and crush injury of the sciatic nerve: a descriptive methodological study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3473253/
https://www.ncbi.nlm.nih.gov/pubmed/22546145
http://dx.doi.org/10.1186/1749-7221-7-5
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