Cargando…
Exploring optimization parameters to increase ssDNA recombineering in Lactococcus lactis and Lactobacillus reuteri
Single-stranded DNA (ssDNA) recombineering is a technology which is used to make subtle changes in the chromosome of several bacterial genera. Cells which express a single-stranded DNA binding protein (RecT or Bet) are transformed with an oligonucleotide which is incorporated via an annealing and re...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Landes Bioscience
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3476877/ https://www.ncbi.nlm.nih.gov/pubmed/22750793 http://dx.doi.org/10.4161/bioe.21049 |