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Exploring optimization parameters to increase ssDNA recombineering in Lactococcus lactis and Lactobacillus reuteri
Single-stranded DNA (ssDNA) recombineering is a technology which is used to make subtle changes in the chromosome of several bacterial genera. Cells which express a single-stranded DNA binding protein (RecT or Bet) are transformed with an oligonucleotide which is incorporated via an annealing and re...
Autores principales: | van Pijkeren, Jan-Peter, Neoh, Kar Mun, Sirias, Denise, Findley, Anthony S., Britton, Robert A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Landes Bioscience
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3476877/ https://www.ncbi.nlm.nih.gov/pubmed/22750793 http://dx.doi.org/10.4161/bioe.21049 |
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