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Bone to pick: the importance of evaluating reference genes for RT-qPCR quantification of gene expression in craniosynostosis and bone-related tissues and cells

BACKGROUND: RT-qPCR is a common tool for quantification of gene expression, but its accuracy is dependent on the choice and stability (steady state expression levels) of the reference gene/s used for normalization. To date, in the bone field, there have been few studies to determine the most stable...

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Detalles Bibliográficos
Autores principales: Yang, Xianxian, Hatfield, Jodie T, Hinze, Susan J, Mu, Xiongzheng, Anderson, Peter J, Powell, Barry C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3476976/
https://www.ncbi.nlm.nih.gov/pubmed/22564426
http://dx.doi.org/10.1186/1756-0500-5-222