Screening for catalytically active Type II restriction endonucleases using segregation-induced methylation deficiency

Type II restriction endonucleases (REases) are one of the basic tools of recombinant DNA technology. They also serve as models for elucidation of mechanisms for both site-specific DNA recognition and cleavage by proteins. However, isolation of catalytically active mutants from their libraries is cha...

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Detalles Bibliográficos
Autores principales: Ukanis, Mindaugas, Sapranauskas, Rimantas, Lubys, Arvydas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479162/
https://www.ncbi.nlm.nih.gov/pubmed/22753027
http://dx.doi.org/10.1093/nar/gks635

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479162/
https://www.ncbi.nlm.nih.gov/pubmed/22753027
http://dx.doi.org/10.1093/nar/gks635

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