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Screening for catalytically active Type II restriction endonucleases using segregation-induced methylation deficiency
Type II restriction endonucleases (REases) are one of the basic tools of recombinant DNA technology. They also serve as models for elucidation of mechanisms for both site-specific DNA recognition and cleavage by proteins. However, isolation of catalytically active mutants from their libraries is cha...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479162/ https://www.ncbi.nlm.nih.gov/pubmed/22753027 http://dx.doi.org/10.1093/nar/gks635 |