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An enzyme-trap approach allows isolation of intermediates in cobalamin biosynthesis

The biosynthesis of many vitamins and coenzymes has often proved difficult to elucidate due to a combination of low abundance and kinetic lability of the pathway intermediates. Through a serial reconstruction of the cobalamin (vitamin B(12)) pathway in E. coli, and by His-tagging the terminal enzyme...

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Detalles Bibliográficos
Autores principales:	Deery, Evelyne, Schroeder, Susanne, Lawrence, Andrew D., Taylor, Samantha L., Seyedarabi, Arefeh, Waterman, Jitka, Wilson, Keith S., Brown, David, Geeves, Michael A., Howard, Mark J., Pickersgill, Richard W., Warren, Martin J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2012
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480714/ https://www.ncbi.nlm.nih.gov/pubmed/23042036 http://dx.doi.org/10.1038/nchembio.1086

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