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Nimesulide inhibits the proliferation of HepG2 by up-regulation of Smad4
OBJECTIVES: Hepatocellular carcinoma (HCC) is receiving increased attention. This study was designed to investigate the effect of selective Cyclooxygenase-2 (COX-2) inhibitor, nimesulide, on the expression of Smad4 in human hepatocellular carcinoma HepG2. MATERIALS AND METHODS: HepG2 cells were incu...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480792/ https://www.ncbi.nlm.nih.gov/pubmed/23112421 http://dx.doi.org/10.4103/0253-7613.100384 |
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author | Yang, Shaoqi Guo, Ruifang Huang, Liya Yang, Li Jiang, Dong |
author_facet | Yang, Shaoqi Guo, Ruifang Huang, Liya Yang, Li Jiang, Dong |
author_sort | Yang, Shaoqi |
collection | PubMed |
description | OBJECTIVES: Hepatocellular carcinoma (HCC) is receiving increased attention. This study was designed to investigate the effect of selective Cyclooxygenase-2 (COX-2) inhibitor, nimesulide, on the expression of Smad4 in human hepatocellular carcinoma HepG2. MATERIALS AND METHODS: HepG2 cells were incubated in various concentrations of nimesulide (25, 50, 100, 200, 400 μmol/L) to detect the effect of proliferation by MTS. The apoptosis of HepG2 was determined by TUNEL; fluorescence microscope was used to observe the expression of Smad4. RESULTS: The result showed that nimesulide inhibited the proliferation of HepG2 cell in a concentrations-dependent manner, and promoted the karyopyknosis and fragmentation of HepG2 cell nucleus, induced its apoptosis, the number of fluorescence labeling of Smad4 in Nimesulide group was higher than control group (P<0.05). CONCLUSIONS: Nimesulide inhibits the proliferation and promotes apoptosis of HepG2 by up-regulation of Smad4 in HepG2. |
format | Online Article Text |
id | pubmed-3480792 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-34807922012-10-30 Nimesulide inhibits the proliferation of HepG2 by up-regulation of Smad4 Yang, Shaoqi Guo, Ruifang Huang, Liya Yang, Li Jiang, Dong Indian J Pharmacol Research Article OBJECTIVES: Hepatocellular carcinoma (HCC) is receiving increased attention. This study was designed to investigate the effect of selective Cyclooxygenase-2 (COX-2) inhibitor, nimesulide, on the expression of Smad4 in human hepatocellular carcinoma HepG2. MATERIALS AND METHODS: HepG2 cells were incubated in various concentrations of nimesulide (25, 50, 100, 200, 400 μmol/L) to detect the effect of proliferation by MTS. The apoptosis of HepG2 was determined by TUNEL; fluorescence microscope was used to observe the expression of Smad4. RESULTS: The result showed that nimesulide inhibited the proliferation of HepG2 cell in a concentrations-dependent manner, and promoted the karyopyknosis and fragmentation of HepG2 cell nucleus, induced its apoptosis, the number of fluorescence labeling of Smad4 in Nimesulide group was higher than control group (P<0.05). CONCLUSIONS: Nimesulide inhibits the proliferation and promotes apoptosis of HepG2 by up-regulation of Smad4 in HepG2. Medknow Publications & Media Pvt Ltd 2012 /pmc/articles/PMC3480792/ /pubmed/23112421 http://dx.doi.org/10.4103/0253-7613.100384 Text en Copyright: © Indian Journal of Pharmacology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yang, Shaoqi Guo, Ruifang Huang, Liya Yang, Li Jiang, Dong Nimesulide inhibits the proliferation of HepG2 by up-regulation of Smad4 |
title | Nimesulide inhibits the proliferation of HepG2 by up-regulation of Smad4 |
title_full | Nimesulide inhibits the proliferation of HepG2 by up-regulation of Smad4 |
title_fullStr | Nimesulide inhibits the proliferation of HepG2 by up-regulation of Smad4 |
title_full_unstemmed | Nimesulide inhibits the proliferation of HepG2 by up-regulation of Smad4 |
title_short | Nimesulide inhibits the proliferation of HepG2 by up-regulation of Smad4 |
title_sort | nimesulide inhibits the proliferation of hepg2 by up-regulation of smad4 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480792/ https://www.ncbi.nlm.nih.gov/pubmed/23112421 http://dx.doi.org/10.4103/0253-7613.100384 |
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