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Nimesulide inhibits the proliferation of HepG2 by up-regulation of Smad4

OBJECTIVES: Hepatocellular carcinoma (HCC) is receiving increased attention. This study was designed to investigate the effect of selective Cyclooxygenase-2 (COX-2) inhibitor, nimesulide, on the expression of Smad4 in human hepatocellular carcinoma HepG2. MATERIALS AND METHODS: HepG2 cells were incu...

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Autores principales: Yang, Shaoqi, Guo, Ruifang, Huang, Liya, Yang, Li, Jiang, Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480792/
https://www.ncbi.nlm.nih.gov/pubmed/23112421
http://dx.doi.org/10.4103/0253-7613.100384
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author Yang, Shaoqi
Guo, Ruifang
Huang, Liya
Yang, Li
Jiang, Dong
author_facet Yang, Shaoqi
Guo, Ruifang
Huang, Liya
Yang, Li
Jiang, Dong
author_sort Yang, Shaoqi
collection PubMed
description OBJECTIVES: Hepatocellular carcinoma (HCC) is receiving increased attention. This study was designed to investigate the effect of selective Cyclooxygenase-2 (COX-2) inhibitor, nimesulide, on the expression of Smad4 in human hepatocellular carcinoma HepG2. MATERIALS AND METHODS: HepG2 cells were incubated in various concentrations of nimesulide (25, 50, 100, 200, 400 μmol/L) to detect the effect of proliferation by MTS. The apoptosis of HepG2 was determined by TUNEL; fluorescence microscope was used to observe the expression of Smad4. RESULTS: The result showed that nimesulide inhibited the proliferation of HepG2 cell in a concentrations-dependent manner, and promoted the karyopyknosis and fragmentation of HepG2 cell nucleus, induced its apoptosis, the number of fluorescence labeling of Smad4 in Nimesulide group was higher than control group (P<0.05). CONCLUSIONS: Nimesulide inhibits the proliferation and promotes apoptosis of HepG2 by up-regulation of Smad4 in HepG2.
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spelling pubmed-34807922012-10-30 Nimesulide inhibits the proliferation of HepG2 by up-regulation of Smad4 Yang, Shaoqi Guo, Ruifang Huang, Liya Yang, Li Jiang, Dong Indian J Pharmacol Research Article OBJECTIVES: Hepatocellular carcinoma (HCC) is receiving increased attention. This study was designed to investigate the effect of selective Cyclooxygenase-2 (COX-2) inhibitor, nimesulide, on the expression of Smad4 in human hepatocellular carcinoma HepG2. MATERIALS AND METHODS: HepG2 cells were incubated in various concentrations of nimesulide (25, 50, 100, 200, 400 μmol/L) to detect the effect of proliferation by MTS. The apoptosis of HepG2 was determined by TUNEL; fluorescence microscope was used to observe the expression of Smad4. RESULTS: The result showed that nimesulide inhibited the proliferation of HepG2 cell in a concentrations-dependent manner, and promoted the karyopyknosis and fragmentation of HepG2 cell nucleus, induced its apoptosis, the number of fluorescence labeling of Smad4 in Nimesulide group was higher than control group (P<0.05). CONCLUSIONS: Nimesulide inhibits the proliferation and promotes apoptosis of HepG2 by up-regulation of Smad4 in HepG2. Medknow Publications & Media Pvt Ltd 2012 /pmc/articles/PMC3480792/ /pubmed/23112421 http://dx.doi.org/10.4103/0253-7613.100384 Text en Copyright: © Indian Journal of Pharmacology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yang, Shaoqi
Guo, Ruifang
Huang, Liya
Yang, Li
Jiang, Dong
Nimesulide inhibits the proliferation of HepG2 by up-regulation of Smad4
title Nimesulide inhibits the proliferation of HepG2 by up-regulation of Smad4
title_full Nimesulide inhibits the proliferation of HepG2 by up-regulation of Smad4
title_fullStr Nimesulide inhibits the proliferation of HepG2 by up-regulation of Smad4
title_full_unstemmed Nimesulide inhibits the proliferation of HepG2 by up-regulation of Smad4
title_short Nimesulide inhibits the proliferation of HepG2 by up-regulation of Smad4
title_sort nimesulide inhibits the proliferation of hepg2 by up-regulation of smad4
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480792/
https://www.ncbi.nlm.nih.gov/pubmed/23112421
http://dx.doi.org/10.4103/0253-7613.100384
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