A Bioinformatics Method Identifies Prominent Off-targeted Transcripts in RNAi Screens

Because off-target effects hamper interpretation and validation of RNAi screens, we developed a bioinformatics method, Genome-wide Enrichment of Seed Sequence matches (GESS), to identify candidate off-targeted transcripts from direct analysis of primary screening data. GESS identified a prominent of...

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Detalles Bibliográficos
Autores principales: Sigoillot, Frederic D., Lyman, Susan, Huckins, Jeremy F., Adamson, Britt, Chung, Eunah, Quattrochi, Brian, King, Randall W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3482495/
https://www.ncbi.nlm.nih.gov/pubmed/22343343
http://dx.doi.org/10.1038/nmeth.1898

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3482495/
https://www.ncbi.nlm.nih.gov/pubmed/22343343
http://dx.doi.org/10.1038/nmeth.1898

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