Cargando…

Splenic Red Pulp Macrophages Produce Type I Interferons as Early Sentinels of Malaria Infection but Are Dispensable for Control

Type I interferons (T1IFNs) are among the earliest cytokines produced during infections due to their direct regulation by innate immune signaling pathways. Reports have suggested that T1IFNs are produced during malaria infection, but little is known about the in vivo cellular origins of T1IFNs or th...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Charles C., Nelson, Christopher S., Wilson, Emily B., Hou, Baidong, DeFranco, Anthony L., DeRisi, Joseph L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483282/
https://www.ncbi.nlm.nih.gov/pubmed/23144737
http://dx.doi.org/10.1371/journal.pone.0048126
_version_ 1782247982519812096
author Kim, Charles C.
Nelson, Christopher S.
Wilson, Emily B.
Hou, Baidong
DeFranco, Anthony L.
DeRisi, Joseph L.
author_facet Kim, Charles C.
Nelson, Christopher S.
Wilson, Emily B.
Hou, Baidong
DeFranco, Anthony L.
DeRisi, Joseph L.
author_sort Kim, Charles C.
collection PubMed
description Type I interferons (T1IFNs) are among the earliest cytokines produced during infections due to their direct regulation by innate immune signaling pathways. Reports have suggested that T1IFNs are produced during malaria infection, but little is known about the in vivo cellular origins of T1IFNs or their role in protection. We have found that in addition to plasmacytoid dendritic cells, splenic red pulp macrophages (RPMs) can generate significant quantities of T1IFNs in response to P. chabaudi infection in a TLR9-, MYD88-, and IRF7-dependent manner. Furthermore, T1IFNs regulate expression of interferon-stimulated genes redundantly with Interferon-gamma (IFNG), resulting in redundancy for resistance to experimental malaria infection. Despite their role in sensing and promoting immune responses to infection, we observe that RPMs are dispensable for control of parasitemia. Our results reveal that RPMs are early sentinels of malaria infection, but that effector mechanisms previously attributed to RPMs are not essential for control.
format Online
Article
Text
id pubmed-3483282
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-34832822012-11-09 Splenic Red Pulp Macrophages Produce Type I Interferons as Early Sentinels of Malaria Infection but Are Dispensable for Control Kim, Charles C. Nelson, Christopher S. Wilson, Emily B. Hou, Baidong DeFranco, Anthony L. DeRisi, Joseph L. PLoS One Research Article Type I interferons (T1IFNs) are among the earliest cytokines produced during infections due to their direct regulation by innate immune signaling pathways. Reports have suggested that T1IFNs are produced during malaria infection, but little is known about the in vivo cellular origins of T1IFNs or their role in protection. We have found that in addition to plasmacytoid dendritic cells, splenic red pulp macrophages (RPMs) can generate significant quantities of T1IFNs in response to P. chabaudi infection in a TLR9-, MYD88-, and IRF7-dependent manner. Furthermore, T1IFNs regulate expression of interferon-stimulated genes redundantly with Interferon-gamma (IFNG), resulting in redundancy for resistance to experimental malaria infection. Despite their role in sensing and promoting immune responses to infection, we observe that RPMs are dispensable for control of parasitemia. Our results reveal that RPMs are early sentinels of malaria infection, but that effector mechanisms previously attributed to RPMs are not essential for control. Public Library of Science 2012-10-29 /pmc/articles/PMC3483282/ /pubmed/23144737 http://dx.doi.org/10.1371/journal.pone.0048126 Text en © 2012 Kim et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kim, Charles C.
Nelson, Christopher S.
Wilson, Emily B.
Hou, Baidong
DeFranco, Anthony L.
DeRisi, Joseph L.
Splenic Red Pulp Macrophages Produce Type I Interferons as Early Sentinels of Malaria Infection but Are Dispensable for Control
title Splenic Red Pulp Macrophages Produce Type I Interferons as Early Sentinels of Malaria Infection but Are Dispensable for Control
title_full Splenic Red Pulp Macrophages Produce Type I Interferons as Early Sentinels of Malaria Infection but Are Dispensable for Control
title_fullStr Splenic Red Pulp Macrophages Produce Type I Interferons as Early Sentinels of Malaria Infection but Are Dispensable for Control
title_full_unstemmed Splenic Red Pulp Macrophages Produce Type I Interferons as Early Sentinels of Malaria Infection but Are Dispensable for Control
title_short Splenic Red Pulp Macrophages Produce Type I Interferons as Early Sentinels of Malaria Infection but Are Dispensable for Control
title_sort splenic red pulp macrophages produce type i interferons as early sentinels of malaria infection but are dispensable for control
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483282/
https://www.ncbi.nlm.nih.gov/pubmed/23144737
http://dx.doi.org/10.1371/journal.pone.0048126
work_keys_str_mv AT kimcharlesc splenicredpulpmacrophagesproducetypeiinterferonsasearlysentinelsofmalariainfectionbutaredispensableforcontrol
AT nelsonchristophers splenicredpulpmacrophagesproducetypeiinterferonsasearlysentinelsofmalariainfectionbutaredispensableforcontrol
AT wilsonemilyb splenicredpulpmacrophagesproducetypeiinterferonsasearlysentinelsofmalariainfectionbutaredispensableforcontrol
AT houbaidong splenicredpulpmacrophagesproducetypeiinterferonsasearlysentinelsofmalariainfectionbutaredispensableforcontrol
AT defrancoanthonyl splenicredpulpmacrophagesproducetypeiinterferonsasearlysentinelsofmalariainfectionbutaredispensableforcontrol
AT derisijosephl splenicredpulpmacrophagesproducetypeiinterferonsasearlysentinelsofmalariainfectionbutaredispensableforcontrol