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Skewed Production of IL-6 and TGFβ by Cultured Salivary Gland Epithelial Cells from Patients with Sjögren's Syndrome
OBJECTIVE: To determine the cytokine production profile of cultured salivary gland epithelial (SGE) cells obtained from patients with Sjögren's syndrome (SS). METHODS: SGE cells obtained from 9 SS patients and 6 normal controls were cultured in the presence of exogenous IFNγ. Cell proliferation...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3484144/ https://www.ncbi.nlm.nih.gov/pubmed/23118848 http://dx.doi.org/10.1371/journal.pone.0045689 |
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author | Kawanami, Takafumi Sawaki, Toshioki Sakai, Tomoyuki Miki, Miyuki Iwao, Haruka Nakajima, Akio Nakamura, Takuji Sato, Tomomi Fujita, Yoshimasa Tanaka, Masao Masaki, Yasufumi Fukushima, Toshihiro Hirose, Yuko Taniguchi, Makoto Sugimoto, Naotoshi Okazaki, Toshiro Umehara, Hisanori |
author_facet | Kawanami, Takafumi Sawaki, Toshioki Sakai, Tomoyuki Miki, Miyuki Iwao, Haruka Nakajima, Akio Nakamura, Takuji Sato, Tomomi Fujita, Yoshimasa Tanaka, Masao Masaki, Yasufumi Fukushima, Toshihiro Hirose, Yuko Taniguchi, Makoto Sugimoto, Naotoshi Okazaki, Toshiro Umehara, Hisanori |
author_sort | Kawanami, Takafumi |
collection | PubMed |
description | OBJECTIVE: To determine the cytokine production profile of cultured salivary gland epithelial (SGE) cells obtained from patients with Sjögren's syndrome (SS). METHODS: SGE cells obtained from 9 SS patients and 6 normal controls were cultured in the presence of exogenous IFNγ. Cell proliferation and apoptosis in response to IFNγ were determined by WST1 assay and by FACS analysis. The concentrations of IL-6 and TGFβ secreted into culture supernatants were analyzed by ELISA. RESULTS: IFNγ did not significantly affect the proliferation or apoptosis of SGE cells. However, IL-6 concentrations were higher, and TGFβ concentrations were lower, in culture supernatants of SGE cells from SS patients than from normal controls. CONCLUSION: Cytokine production by SGE cells from SS patients showed a skewed balance compared with normal controls, with increased IL-6 and decreased TGFβ secretion. This imbalance may be critical in the regulation of Treg/Th17 cells and may foster a pathogenic milieu that may be causative and predictive in SS. |
format | Online Article Text |
id | pubmed-3484144 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34841442012-11-01 Skewed Production of IL-6 and TGFβ by Cultured Salivary Gland Epithelial Cells from Patients with Sjögren's Syndrome Kawanami, Takafumi Sawaki, Toshioki Sakai, Tomoyuki Miki, Miyuki Iwao, Haruka Nakajima, Akio Nakamura, Takuji Sato, Tomomi Fujita, Yoshimasa Tanaka, Masao Masaki, Yasufumi Fukushima, Toshihiro Hirose, Yuko Taniguchi, Makoto Sugimoto, Naotoshi Okazaki, Toshiro Umehara, Hisanori PLoS One Research Article OBJECTIVE: To determine the cytokine production profile of cultured salivary gland epithelial (SGE) cells obtained from patients with Sjögren's syndrome (SS). METHODS: SGE cells obtained from 9 SS patients and 6 normal controls were cultured in the presence of exogenous IFNγ. Cell proliferation and apoptosis in response to IFNγ were determined by WST1 assay and by FACS analysis. The concentrations of IL-6 and TGFβ secreted into culture supernatants were analyzed by ELISA. RESULTS: IFNγ did not significantly affect the proliferation or apoptosis of SGE cells. However, IL-6 concentrations were higher, and TGFβ concentrations were lower, in culture supernatants of SGE cells from SS patients than from normal controls. CONCLUSION: Cytokine production by SGE cells from SS patients showed a skewed balance compared with normal controls, with increased IL-6 and decreased TGFβ secretion. This imbalance may be critical in the regulation of Treg/Th17 cells and may foster a pathogenic milieu that may be causative and predictive in SS. Public Library of Science 2012-10-30 /pmc/articles/PMC3484144/ /pubmed/23118848 http://dx.doi.org/10.1371/journal.pone.0045689 Text en © 2012 Kawanami et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kawanami, Takafumi Sawaki, Toshioki Sakai, Tomoyuki Miki, Miyuki Iwao, Haruka Nakajima, Akio Nakamura, Takuji Sato, Tomomi Fujita, Yoshimasa Tanaka, Masao Masaki, Yasufumi Fukushima, Toshihiro Hirose, Yuko Taniguchi, Makoto Sugimoto, Naotoshi Okazaki, Toshiro Umehara, Hisanori Skewed Production of IL-6 and TGFβ by Cultured Salivary Gland Epithelial Cells from Patients with Sjögren's Syndrome |
title | Skewed Production of IL-6 and TGFβ by Cultured Salivary Gland Epithelial Cells from Patients with Sjögren's Syndrome |
title_full | Skewed Production of IL-6 and TGFβ by Cultured Salivary Gland Epithelial Cells from Patients with Sjögren's Syndrome |
title_fullStr | Skewed Production of IL-6 and TGFβ by Cultured Salivary Gland Epithelial Cells from Patients with Sjögren's Syndrome |
title_full_unstemmed | Skewed Production of IL-6 and TGFβ by Cultured Salivary Gland Epithelial Cells from Patients with Sjögren's Syndrome |
title_short | Skewed Production of IL-6 and TGFβ by Cultured Salivary Gland Epithelial Cells from Patients with Sjögren's Syndrome |
title_sort | skewed production of il-6 and tgfβ by cultured salivary gland epithelial cells from patients with sjögren's syndrome |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3484144/ https://www.ncbi.nlm.nih.gov/pubmed/23118848 http://dx.doi.org/10.1371/journal.pone.0045689 |
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