A Cell-Based Method for Screening RNA-Protein Interactions: Identification of Constitutive Transport Element-Interacting Proteins

We have developed a mammalian cell-based screening platform to identify proteins that assemble into RNA-protein complexes. Based on Tat-mediated activation of the HIV LTR, proteins that interact with an RNA target elicit expression of a GFP reporter and are captured by fluorescence activated cell so...

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Detalles Bibliográficos
Autores principales: Nakamura, Robert L., Landt, Stephen G., Mai, Emily, Nejim, Jemiel, Chen, Lily, Frankel, Alan D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3485056/
https://www.ncbi.nlm.nih.gov/pubmed/23133567
http://dx.doi.org/10.1371/journal.pone.0048194

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3485056/
https://www.ncbi.nlm.nih.gov/pubmed/23133567
http://dx.doi.org/10.1371/journal.pone.0048194

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