A Dual Reporter Splicing Assay Using HaloTag-containing Proteins

To evaluate the effects of genetic variations on mRNA splicing, we developed a minigene-based splicing assay using reporter genes encoding luciferase and the multifunctional HaloTag protein. In addition to conventional RT-PCR analysis, splicing events can be monitored in this system using two parame...

Descripción completa

Detalles Bibliográficos
Autores principales: Oshima, Koichi, Nagase, Takahiro, Imai, Kohsuke, Nonoyama, Shigeaki, Obara, Megumi, Mizukami, Tomoyuki, Nunoi, Hiroyuki, Kanegane, Hirokazu, Kuribayashi, Futoshi, Amemiya, Shin, Ohara, Osamu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3486960/
https://www.ncbi.nlm.nih.gov/pubmed/23136623
http://dx.doi.org/10.2174/1875397301206010027

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3486960/
https://www.ncbi.nlm.nih.gov/pubmed/23136623
http://dx.doi.org/10.2174/1875397301206010027

Ejemplares similares