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A Dual Reporter Splicing Assay Using HaloTag-containing Proteins

To evaluate the effects of genetic variations on mRNA splicing, we developed a minigene-based splicing assay using reporter genes encoding luciferase and the multifunctional HaloTag protein. In addition to conventional RT-PCR analysis, splicing events can be monitored in this system using two parame...

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Detalles Bibliográficos
Autores principales:	Oshima, Koichi, Nagase, Takahiro, Imai, Kohsuke, Nonoyama, Shigeaki, Obara, Megumi, Mizukami, Tomoyuki, Nunoi, Hiroyuki, Kanegane, Hirokazu, Kuribayashi, Futoshi, Amemiya, Shin, Ohara, Osamu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Bentham Open 2012
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3486960/ https://www.ncbi.nlm.nih.gov/pubmed/23136623 http://dx.doi.org/10.2174/1875397301206010027

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