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A sensitive method for rapid detection of alkyl halides and dehalogenase activity using a multistep enzyme assay
A method for the detection of haloalkane conversion to the corresponding alcohols by haloalkane dehalogenases is described. It is based on a multistage enzyme reaction which allows for the analysis of alkyl halides in buffered systems. Irreversible hydrolytic dehalogenation catalyzed by haloalkane d...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3487978/ https://www.ncbi.nlm.nih.gov/pubmed/23006907 http://dx.doi.org/10.1186/2191-0855-2-51 |