Response of C57Bl/6 mice to a carbohydrate-free diet

High fat feeding in rodents generally leads to obesity and insulin resistance whereas in humans this is only seen if dietary carbohydrate is also high, the result of the anabolic effect of poor regulation of glucose and insulin. A previous study of C57Bl/6 mice (Kennedy AR, et al.: Am J Physiol Endo...

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Autores principales: Borghjid, Saihan, Feinman, Richard David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3488544/
https://www.ncbi.nlm.nih.gov/pubmed/22838969
http://dx.doi.org/10.1186/1743-7075-9-69
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author Borghjid, Saihan
Feinman, Richard David
author_facet Borghjid, Saihan
Feinman, Richard David
author_sort Borghjid, Saihan
collection PubMed
description High fat feeding in rodents generally leads to obesity and insulin resistance whereas in humans this is only seen if dietary carbohydrate is also high, the result of the anabolic effect of poor regulation of glucose and insulin. A previous study of C57Bl/6 mice (Kennedy AR, et al.: Am J Physiol Endocrinol Metab (2007) 262 E1724-1739) appeared to show the kind of beneficial effects of calorie restriction that is seen in humans but that diet was unusually low in protein (5%). In the current study, we tested a zero-carbohydrate diet that had a higher protein content (20%). Mice on the zero-carbohydrate diet, despite similar caloric intake, consistently gained more weight than animals consuming standard chow, attaining a dramatic difference by week 16 (46.1 ± 1.38 g vs. 30.4 ± 1.00 g for the chow group). Consistent with the obese phenotype, experimental mice had fatty livers and hearts as well as large fat deposits in the abdomino-pelvic cavity, and showed impaired glucose clearance after intraperitoneal injection. In sum, the response of mice to a carbohydrate-free diet was greater weight gain and metabolic disruptions in distinction to the response in humans where low carbohydrate diets cause greater weight loss than isocaloric controls. The results suggest that rodent models of obesity may be most valuable in the understanding of how metabolic mechanisms can work in ways different from the effect in humans.
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spelling pubmed-34885442012-11-05 Response of C57Bl/6 mice to a carbohydrate-free diet Borghjid, Saihan Feinman, Richard David Nutr Metab (Lond) Research High fat feeding in rodents generally leads to obesity and insulin resistance whereas in humans this is only seen if dietary carbohydrate is also high, the result of the anabolic effect of poor regulation of glucose and insulin. A previous study of C57Bl/6 mice (Kennedy AR, et al.: Am J Physiol Endocrinol Metab (2007) 262 E1724-1739) appeared to show the kind of beneficial effects of calorie restriction that is seen in humans but that diet was unusually low in protein (5%). In the current study, we tested a zero-carbohydrate diet that had a higher protein content (20%). Mice on the zero-carbohydrate diet, despite similar caloric intake, consistently gained more weight than animals consuming standard chow, attaining a dramatic difference by week 16 (46.1 ± 1.38 g vs. 30.4 ± 1.00 g for the chow group). Consistent with the obese phenotype, experimental mice had fatty livers and hearts as well as large fat deposits in the abdomino-pelvic cavity, and showed impaired glucose clearance after intraperitoneal injection. In sum, the response of mice to a carbohydrate-free diet was greater weight gain and metabolic disruptions in distinction to the response in humans where low carbohydrate diets cause greater weight loss than isocaloric controls. The results suggest that rodent models of obesity may be most valuable in the understanding of how metabolic mechanisms can work in ways different from the effect in humans. BioMed Central 2012-07-28 /pmc/articles/PMC3488544/ /pubmed/22838969 http://dx.doi.org/10.1186/1743-7075-9-69 Text en Copyright ©2012 Borghjid and Feinman; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Borghjid, Saihan
Feinman, Richard David
Response of C57Bl/6 mice to a carbohydrate-free diet
title Response of C57Bl/6 mice to a carbohydrate-free diet
title_full Response of C57Bl/6 mice to a carbohydrate-free diet
title_fullStr Response of C57Bl/6 mice to a carbohydrate-free diet
title_full_unstemmed Response of C57Bl/6 mice to a carbohydrate-free diet
title_short Response of C57Bl/6 mice to a carbohydrate-free diet
title_sort response of c57bl/6 mice to a carbohydrate-free diet
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3488544/
https://www.ncbi.nlm.nih.gov/pubmed/22838969
http://dx.doi.org/10.1186/1743-7075-9-69
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