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Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease

The interrogation of beta cell gene expression and function in vitro has squarely shifted over the years from the study of rodent tumorigenic cell lines to the study of isolated rodent islets. Primary islets offer the distinct advantage that they more faithfully reflect the biology of intracellular...

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Detalles Bibliográficos
Autores principales: Stull, Natalie D., Breite, Andrew, McCarthy, Robert, Tersey, Sarah A., Mirmira, Raghavendra G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490250/
https://www.ncbi.nlm.nih.gov/pubmed/22987198
http://dx.doi.org/10.3791/4137