A Protocol for the Identification of Protein-protein Interactions Based on (15)N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

Protein-protein interactions are fundamental for many biological processes in the cell. Therefore, their characterization plays an important role in current research and a plethora of methods for their investigation is available(1). Protein-protein interactions often are highly dynamic and may depen...

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Detalles Bibliográficos
Autores principales: Schmollinger, Stefan, Strenkert, Daniela, Offeddu, Vittoria, Nordhues, André, Sommer, Frederik, Schroda, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2012
Materias:
Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490270/
https://www.ncbi.nlm.nih.gov/pubmed/23051728
http://dx.doi.org/10.3791/4083

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490270/
https://www.ncbi.nlm.nih.gov/pubmed/23051728
http://dx.doi.org/10.3791/4083

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