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A Protocol for the Identification of Protein-protein Interactions Based on (15)N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation
Protein-protein interactions are fundamental for many biological processes in the cell. Therefore, their characterization plays an important role in current research and a plethora of methods for their investigation is available(1). Protein-protein interactions often are highly dynamic and may depen...
Autores principales: | Schmollinger, Stefan, Strenkert, Daniela, Offeddu, Vittoria, Nordhues, André, Sommer, Frederik, Schroda, Michael |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490270/ https://www.ncbi.nlm.nih.gov/pubmed/23051728 http://dx.doi.org/10.3791/4083 |
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