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Role of MicroRNAs 99b, 181a, and 181b in the Differentiation of Human Embryonic Stem Cells to Vascular Endothelial Cells

MicroRNAs (miRNAs) are short noncoding RNAs, which post-transcriptionally regulate gene expression. miRNAs are transcribed as precursors and matured to active forms by a series of enzymes, including Dicer. miRNAs are important in governing cell differentiation, development, and disease. We have rece...

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Autores principales: Kane, Nicole M, Howard, Lynsey, Descamps, Betty, Meloni, Marco, McClure, John, Lu, Ruifang, McCahill, Angela, Breen, Christopher, Mackenzie, Ruth M, Delles, Christian, Mountford, Joanne C, Milligan, Graeme, Emanueli, Costanza, Baker, Andrew H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wiley Subscription Services, Inc., A Wiley Company 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490385/
https://www.ncbi.nlm.nih.gov/pubmed/22232059
http://dx.doi.org/10.1002/stem.1026
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author Kane, Nicole M
Howard, Lynsey
Descamps, Betty
Meloni, Marco
McClure, John
Lu, Ruifang
McCahill, Angela
Breen, Christopher
Mackenzie, Ruth M
Delles, Christian
Mountford, Joanne C
Milligan, Graeme
Emanueli, Costanza
Baker, Andrew H
author_facet Kane, Nicole M
Howard, Lynsey
Descamps, Betty
Meloni, Marco
McClure, John
Lu, Ruifang
McCahill, Angela
Breen, Christopher
Mackenzie, Ruth M
Delles, Christian
Mountford, Joanne C
Milligan, Graeme
Emanueli, Costanza
Baker, Andrew H
author_sort Kane, Nicole M
collection PubMed
description MicroRNAs (miRNAs) are short noncoding RNAs, which post-transcriptionally regulate gene expression. miRNAs are transcribed as precursors and matured to active forms by a series of enzymes, including Dicer. miRNAs are important in governing cell differentiation, development, and disease. We have recently developed a feeder- and serum-free protocol for direct derivation of endothelial cells (ECs) from human embryonic stem cells (hESCs) and provided evidence of increases in angiogenesis-associated miRNAs (miR-126 and -210) during the process. However, the functional role of miRNAs in hESC differentiation to vascular EC remains to be fully interrogated. Here, we show that the reduction of miRNA maturation induced by Dicer knockdown suppressed hES-EC differentiation. A miRNA microarray was performed to quantify hES-EC miRNA profiles during defined stages of endothelial differentiation. miR-99b, -181a, and -181b were identified as increasing in a time- and differentiation-dependent manner to peak in mature hESC-ECs and adult ECs. Augmentation of miR-99b, -181a, and -181b levels by lentiviral-mediated transfer potentiated the mRNA and protein expression of EC-specific markers, Pecam1 and VE Cadherin, increased nitric oxide production, and improved hES-EC-induced therapeutic neovascularization in vivo. Conversely, knockdown did not impact endothelial differentiation. Our results suggest that miR-99b, -181a, and -181b comprise a component of an endothelial-miRNA signature and are capable of potentiating EC differentiation from pluripotent hESCs
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spelling pubmed-34903852012-11-08 Role of MicroRNAs 99b, 181a, and 181b in the Differentiation of Human Embryonic Stem Cells to Vascular Endothelial Cells Kane, Nicole M Howard, Lynsey Descamps, Betty Meloni, Marco McClure, John Lu, Ruifang McCahill, Angela Breen, Christopher Mackenzie, Ruth M Delles, Christian Mountford, Joanne C Milligan, Graeme Emanueli, Costanza Baker, Andrew H Stem Cells Embryonic Stem Cells/Induced Pluripotent Stem Cells MicroRNAs (miRNAs) are short noncoding RNAs, which post-transcriptionally regulate gene expression. miRNAs are transcribed as precursors and matured to active forms by a series of enzymes, including Dicer. miRNAs are important in governing cell differentiation, development, and disease. We have recently developed a feeder- and serum-free protocol for direct derivation of endothelial cells (ECs) from human embryonic stem cells (hESCs) and provided evidence of increases in angiogenesis-associated miRNAs (miR-126 and -210) during the process. However, the functional role of miRNAs in hESC differentiation to vascular EC remains to be fully interrogated. Here, we show that the reduction of miRNA maturation induced by Dicer knockdown suppressed hES-EC differentiation. A miRNA microarray was performed to quantify hES-EC miRNA profiles during defined stages of endothelial differentiation. miR-99b, -181a, and -181b were identified as increasing in a time- and differentiation-dependent manner to peak in mature hESC-ECs and adult ECs. Augmentation of miR-99b, -181a, and -181b levels by lentiviral-mediated transfer potentiated the mRNA and protein expression of EC-specific markers, Pecam1 and VE Cadherin, increased nitric oxide production, and improved hES-EC-induced therapeutic neovascularization in vivo. Conversely, knockdown did not impact endothelial differentiation. Our results suggest that miR-99b, -181a, and -181b comprise a component of an endothelial-miRNA signature and are capable of potentiating EC differentiation from pluripotent hESCs Wiley Subscription Services, Inc., A Wiley Company 2012-04 2012-01-09 /pmc/articles/PMC3490385/ /pubmed/22232059 http://dx.doi.org/10.1002/stem.1026 Text en Copyright © 2012 AlphaMed Press http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Embryonic Stem Cells/Induced Pluripotent Stem Cells
Kane, Nicole M
Howard, Lynsey
Descamps, Betty
Meloni, Marco
McClure, John
Lu, Ruifang
McCahill, Angela
Breen, Christopher
Mackenzie, Ruth M
Delles, Christian
Mountford, Joanne C
Milligan, Graeme
Emanueli, Costanza
Baker, Andrew H
Role of MicroRNAs 99b, 181a, and 181b in the Differentiation of Human Embryonic Stem Cells to Vascular Endothelial Cells
title Role of MicroRNAs 99b, 181a, and 181b in the Differentiation of Human Embryonic Stem Cells to Vascular Endothelial Cells
title_full Role of MicroRNAs 99b, 181a, and 181b in the Differentiation of Human Embryonic Stem Cells to Vascular Endothelial Cells
title_fullStr Role of MicroRNAs 99b, 181a, and 181b in the Differentiation of Human Embryonic Stem Cells to Vascular Endothelial Cells
title_full_unstemmed Role of MicroRNAs 99b, 181a, and 181b in the Differentiation of Human Embryonic Stem Cells to Vascular Endothelial Cells
title_short Role of MicroRNAs 99b, 181a, and 181b in the Differentiation of Human Embryonic Stem Cells to Vascular Endothelial Cells
title_sort role of micrornas 99b, 181a, and 181b in the differentiation of human embryonic stem cells to vascular endothelial cells
topic Embryonic Stem Cells/Induced Pluripotent Stem Cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490385/
https://www.ncbi.nlm.nih.gov/pubmed/22232059
http://dx.doi.org/10.1002/stem.1026
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