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Next-Generation cDNA Screening for Oncogene and Resistance Phenotypes
There is a pressing need for methods to define the functional relevance of genetic alterations identified by next-generation sequencing of cancer specimens. We developed new approaches to efficiently construct full-length cDNA libraries from small amounts of total RNA, screen for transforming and re...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492311/ https://www.ncbi.nlm.nih.gov/pubmed/23145123 http://dx.doi.org/10.1371/journal.pone.0049201 |