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Multi-modal microtubule binding by the Ndc80 kinetochore complex
The Ndc80 complex is a key site of kinetochore-microtubule attachment during cell division. The human complex engages microtubules with a globular “head” formed by tandem calponin-homology domains and an 80 amino-acid unstructured “tail” that contains sites of phospho-regulation by the Aurora B kina...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492541/ https://www.ncbi.nlm.nih.gov/pubmed/23085714 http://dx.doi.org/10.1038/nsmb.2411 |
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author | Alushin, Gregory M. Musinipally, Vivek Matson, Daniel Tooley, John Stukenberg, P. Todd Nogales, Eva |
author_facet | Alushin, Gregory M. Musinipally, Vivek Matson, Daniel Tooley, John Stukenberg, P. Todd Nogales, Eva |
author_sort | Alushin, Gregory M. |
collection | PubMed |
description | The Ndc80 complex is a key site of kinetochore-microtubule attachment during cell division. The human complex engages microtubules with a globular “head” formed by tandem calponin-homology domains and an 80 amino-acid unstructured “tail” that contains sites of phospho-regulation by the Aurora B kinase. Using biochemical, cell biological, and electron microscopy analyses, we have dissected the tail’s roles in microtubule binding and mediating cooperative interactions between Ndc80 complexes. Two segments of the tail that contain Aurora B sites become ordered at interfaces; one with tubulin and the second with an adjacent Ndc80 head on the microtubule surface, forming interactions which are disrupted by phosphorylation. We propose a model in which Ndc80’s interaction with either growing or shrinking microtubule ends can be tuned by the phosphorylation state of its tail. |
format | Online Article Text |
id | pubmed-3492541 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
record_format | MEDLINE/PubMed |
spelling | pubmed-34925412013-05-01 Multi-modal microtubule binding by the Ndc80 kinetochore complex Alushin, Gregory M. Musinipally, Vivek Matson, Daniel Tooley, John Stukenberg, P. Todd Nogales, Eva Nat Struct Mol Biol Article The Ndc80 complex is a key site of kinetochore-microtubule attachment during cell division. The human complex engages microtubules with a globular “head” formed by tandem calponin-homology domains and an 80 amino-acid unstructured “tail” that contains sites of phospho-regulation by the Aurora B kinase. Using biochemical, cell biological, and electron microscopy analyses, we have dissected the tail’s roles in microtubule binding and mediating cooperative interactions between Ndc80 complexes. Two segments of the tail that contain Aurora B sites become ordered at interfaces; one with tubulin and the second with an adjacent Ndc80 head on the microtubule surface, forming interactions which are disrupted by phosphorylation. We propose a model in which Ndc80’s interaction with either growing or shrinking microtubule ends can be tuned by the phosphorylation state of its tail. 2012-10-21 2012-11 /pmc/articles/PMC3492541/ /pubmed/23085714 http://dx.doi.org/10.1038/nsmb.2411 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Alushin, Gregory M. Musinipally, Vivek Matson, Daniel Tooley, John Stukenberg, P. Todd Nogales, Eva Multi-modal microtubule binding by the Ndc80 kinetochore complex |
title | Multi-modal microtubule binding by the Ndc80 kinetochore complex |
title_full | Multi-modal microtubule binding by the Ndc80 kinetochore complex |
title_fullStr | Multi-modal microtubule binding by the Ndc80 kinetochore complex |
title_full_unstemmed | Multi-modal microtubule binding by the Ndc80 kinetochore complex |
title_short | Multi-modal microtubule binding by the Ndc80 kinetochore complex |
title_sort | multi-modal microtubule binding by the ndc80 kinetochore complex |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492541/ https://www.ncbi.nlm.nih.gov/pubmed/23085714 http://dx.doi.org/10.1038/nsmb.2411 |
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