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Metabolomics reveals the metabolic shifts following an intervention with rye bread in postmenopausal women- a randomized control trial

BACKGROUND: Epidemiological studies have consistently shown that whole grain (WG) cereals can protect against the development of chronic diseases, but the underlying mechanism is not fully understood. Among WG products, WG rye is considered even more potent because of its unique discrepancy in postp...

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Autores principales: Moazzami, Ali A, Bondia-Pons, Isabel, Hanhineva, Kati, Juntunen, Katri, Antl, Nadja, Poutanen, Kaisa, Mykkänen, Hannu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493305/
https://www.ncbi.nlm.nih.gov/pubmed/23088297
http://dx.doi.org/10.1186/1475-2891-11-88
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author Moazzami, Ali A
Bondia-Pons, Isabel
Hanhineva, Kati
Juntunen, Katri
Antl, Nadja
Poutanen, Kaisa
Mykkänen, Hannu
author_facet Moazzami, Ali A
Bondia-Pons, Isabel
Hanhineva, Kati
Juntunen, Katri
Antl, Nadja
Poutanen, Kaisa
Mykkänen, Hannu
author_sort Moazzami, Ali A
collection PubMed
description BACKGROUND: Epidemiological studies have consistently shown that whole grain (WG) cereals can protect against the development of chronic diseases, but the underlying mechanism is not fully understood. Among WG products, WG rye is considered even more potent because of its unique discrepancy in postprandial insulin and glucose responses known as the rye factor. In this study, an NMR-based metabolomics approach was applied to study the metabolic effects of WG rye as a tool to determine the beneficial effects of WG rye on human health. METHODS: Thirty-three postmenopausal Finnish women with elevated serum total cholesterol (5.0-8.5 mmol/L) and BMI of 20–33 kg/m(2) consumed a minimum of 20% of their daily energy intake as high fiber WG rye bread (RB) or refined wheat bread (WB) in a randomized, controlled, crossover design with two 8-wk intervention periods separated by an 8-wk washout period. At the end of each intervention period, fasting serum was collected for NMR-based metabolomics and the analysis of cholesterol fractions. Multilevel partial least squares discriminant analysis was used for paired comparisons of multivariate data. RESULTS: The metabolomics analysis of serum showed lower leucine and isoleucine and higher betaine and N,N-dimethylglycine levels after RB than WB intake. To further investigate the metabolic effects of RB, the serum cholesterol fractions were measured. Total- and LDL-cholesterol levels were higher after RB intake than after WB (p<0.05). CONCLUSIONS: This study revealed favorable shifts in branched amino acid and single carbon metabolism and an unfavorable shift in serum cholesterol levels after RB intake in postmenopausal women, which should be considered for evaluating health beneficial effects of rye products.
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spelling pubmed-34933052012-11-09 Metabolomics reveals the metabolic shifts following an intervention with rye bread in postmenopausal women- a randomized control trial Moazzami, Ali A Bondia-Pons, Isabel Hanhineva, Kati Juntunen, Katri Antl, Nadja Poutanen, Kaisa Mykkänen, Hannu Nutr J Research BACKGROUND: Epidemiological studies have consistently shown that whole grain (WG) cereals can protect against the development of chronic diseases, but the underlying mechanism is not fully understood. Among WG products, WG rye is considered even more potent because of its unique discrepancy in postprandial insulin and glucose responses known as the rye factor. In this study, an NMR-based metabolomics approach was applied to study the metabolic effects of WG rye as a tool to determine the beneficial effects of WG rye on human health. METHODS: Thirty-three postmenopausal Finnish women with elevated serum total cholesterol (5.0-8.5 mmol/L) and BMI of 20–33 kg/m(2) consumed a minimum of 20% of their daily energy intake as high fiber WG rye bread (RB) or refined wheat bread (WB) in a randomized, controlled, crossover design with two 8-wk intervention periods separated by an 8-wk washout period. At the end of each intervention period, fasting serum was collected for NMR-based metabolomics and the analysis of cholesterol fractions. Multilevel partial least squares discriminant analysis was used for paired comparisons of multivariate data. RESULTS: The metabolomics analysis of serum showed lower leucine and isoleucine and higher betaine and N,N-dimethylglycine levels after RB than WB intake. To further investigate the metabolic effects of RB, the serum cholesterol fractions were measured. Total- and LDL-cholesterol levels were higher after RB intake than after WB (p<0.05). CONCLUSIONS: This study revealed favorable shifts in branched amino acid and single carbon metabolism and an unfavorable shift in serum cholesterol levels after RB intake in postmenopausal women, which should be considered for evaluating health beneficial effects of rye products. BioMed Central 2012-10-22 /pmc/articles/PMC3493305/ /pubmed/23088297 http://dx.doi.org/10.1186/1475-2891-11-88 Text en Copyright ©2012 Moazzami et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Moazzami, Ali A
Bondia-Pons, Isabel
Hanhineva, Kati
Juntunen, Katri
Antl, Nadja
Poutanen, Kaisa
Mykkänen, Hannu
Metabolomics reveals the metabolic shifts following an intervention with rye bread in postmenopausal women- a randomized control trial
title Metabolomics reveals the metabolic shifts following an intervention with rye bread in postmenopausal women- a randomized control trial
title_full Metabolomics reveals the metabolic shifts following an intervention with rye bread in postmenopausal women- a randomized control trial
title_fullStr Metabolomics reveals the metabolic shifts following an intervention with rye bread in postmenopausal women- a randomized control trial
title_full_unstemmed Metabolomics reveals the metabolic shifts following an intervention with rye bread in postmenopausal women- a randomized control trial
title_short Metabolomics reveals the metabolic shifts following an intervention with rye bread in postmenopausal women- a randomized control trial
title_sort metabolomics reveals the metabolic shifts following an intervention with rye bread in postmenopausal women- a randomized control trial
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493305/
https://www.ncbi.nlm.nih.gov/pubmed/23088297
http://dx.doi.org/10.1186/1475-2891-11-88
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