Using Multiple Phenotype Assays and Epistasis Testing to Enhance the Reliability of RNAi Screening and Identify Regulators of Muscle Protein Degradation

RNAi is a convenient, widely used tool for screening for genes of interest. We have recently used this technology to screen roughly 750 candidate genes, in C. elegans, for potential roles in regulating muscle protein degradation in vivo. To maximize confidence and assess reproducibility, we have onl...

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Detalles Bibliográficos
Autores principales: Lehmann, Susann, Shephard, Freya, Jacobson, Lewis A., Szewczyk, Nathaniel J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495584/
https://www.ncbi.nlm.nih.gov/pubmed/23152949
http://dx.doi.org/10.3390/genes3040686

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495584/
https://www.ncbi.nlm.nih.gov/pubmed/23152949
http://dx.doi.org/10.3390/genes3040686

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