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An improved allele-specific PCR primer design method for SNP marker analysis and its application

BACKGROUND: Although Single Nucleotide Polymorphism (SNP) marker is an invaluable tool for positional cloning, association study and evolutionary analysis, low SNP detection efficiency by Allele-Specific PCR (AS-PCR) still restricts its application as molecular marker like other markers such as Simp...

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Detalles Bibliográficos
Autores principales: Liu, Jing, Huang, Shunmou, Sun, Meiyu, Liu, Shengyi, Liu, Yumei, Wang, Wanxing, Zhang, Xiurong, Wang, Hanzhong, Hua, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495711/
https://www.ncbi.nlm.nih.gov/pubmed/22920499
http://dx.doi.org/10.1186/1746-4811-8-34