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APOBEC3B can impair genomic stability by inducing base substitutions in genomic DNA in human cells

Human APOBEC3 proteins play pivotal roles in intracellular defense against viral infection by catalyzing deamination of cytidine residues, leading to base substitutions in viral DNA. Activation-induced cytidine deaminase (AID), another member of the APOBEC family, is capable of editing immunoglobuli...

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Autores principales: Shinohara, Masanobu, Io, Katsuhiro, Shindo, Keisuke, Matsui, Masashi, Sakamoto, Takashi, Tada, Kohei, Kobayashi, Masayuki, Kadowaki, Norimitsu, Takaori-Kondo, Akifumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3496164/
https://www.ncbi.nlm.nih.gov/pubmed/23150777
http://dx.doi.org/10.1038/srep00806
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author Shinohara, Masanobu
Io, Katsuhiro
Shindo, Keisuke
Matsui, Masashi
Sakamoto, Takashi
Tada, Kohei
Kobayashi, Masayuki
Kadowaki, Norimitsu
Takaori-Kondo, Akifumi
author_facet Shinohara, Masanobu
Io, Katsuhiro
Shindo, Keisuke
Matsui, Masashi
Sakamoto, Takashi
Tada, Kohei
Kobayashi, Masayuki
Kadowaki, Norimitsu
Takaori-Kondo, Akifumi
author_sort Shinohara, Masanobu
collection PubMed
description Human APOBEC3 proteins play pivotal roles in intracellular defense against viral infection by catalyzing deamination of cytidine residues, leading to base substitutions in viral DNA. Activation-induced cytidine deaminase (AID), another member of the APOBEC family, is capable of editing immunoglobulin (Ig) and non-Ig genes, and aberrant expression of AID leads to tumorigenesis. However, it remains unclear whether APOBEC3 (A3) proteins affect stability of human genome. Here we demonstrate that both A3A and A3B can induce base substitutions into human genome as AID can. A3B is highly expressed in several lymphoma cells and somatic mutations occur in some oncogenes of the cells highly expressing A3B. Furthermore, transfection of A3B gene into lymphoma cells induces base substitutions in cMYC gene. These data suggest that aberrant expression of A3B can evoke genomic instability by inducing base substitutions into human genome, which might lead to tumorigenesis in human cells.
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spelling pubmed-34961642012-11-13 APOBEC3B can impair genomic stability by inducing base substitutions in genomic DNA in human cells Shinohara, Masanobu Io, Katsuhiro Shindo, Keisuke Matsui, Masashi Sakamoto, Takashi Tada, Kohei Kobayashi, Masayuki Kadowaki, Norimitsu Takaori-Kondo, Akifumi Sci Rep Article Human APOBEC3 proteins play pivotal roles in intracellular defense against viral infection by catalyzing deamination of cytidine residues, leading to base substitutions in viral DNA. Activation-induced cytidine deaminase (AID), another member of the APOBEC family, is capable of editing immunoglobulin (Ig) and non-Ig genes, and aberrant expression of AID leads to tumorigenesis. However, it remains unclear whether APOBEC3 (A3) proteins affect stability of human genome. Here we demonstrate that both A3A and A3B can induce base substitutions into human genome as AID can. A3B is highly expressed in several lymphoma cells and somatic mutations occur in some oncogenes of the cells highly expressing A3B. Furthermore, transfection of A3B gene into lymphoma cells induces base substitutions in cMYC gene. These data suggest that aberrant expression of A3B can evoke genomic instability by inducing base substitutions into human genome, which might lead to tumorigenesis in human cells. Nature Publishing Group 2012-11-13 /pmc/articles/PMC3496164/ /pubmed/23150777 http://dx.doi.org/10.1038/srep00806 Text en Copyright © 2012, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Article
Shinohara, Masanobu
Io, Katsuhiro
Shindo, Keisuke
Matsui, Masashi
Sakamoto, Takashi
Tada, Kohei
Kobayashi, Masayuki
Kadowaki, Norimitsu
Takaori-Kondo, Akifumi
APOBEC3B can impair genomic stability by inducing base substitutions in genomic DNA in human cells
title APOBEC3B can impair genomic stability by inducing base substitutions in genomic DNA in human cells
title_full APOBEC3B can impair genomic stability by inducing base substitutions in genomic DNA in human cells
title_fullStr APOBEC3B can impair genomic stability by inducing base substitutions in genomic DNA in human cells
title_full_unstemmed APOBEC3B can impair genomic stability by inducing base substitutions in genomic DNA in human cells
title_short APOBEC3B can impair genomic stability by inducing base substitutions in genomic DNA in human cells
title_sort apobec3b can impair genomic stability by inducing base substitutions in genomic dna in human cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3496164/
https://www.ncbi.nlm.nih.gov/pubmed/23150777
http://dx.doi.org/10.1038/srep00806
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