One-step purification of assembly-competent tubulin from diverse eukaryotic sources

We have developed a protocol that allows rapid and efficient purification of native, active tubulin from a variety of species and tissue sources by affinity chromatography. The affinity matrix comprises a bacterially expressed, recombinant protein, the TOG1/2 domains from Saccharomyces cerevisiae St...

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Detalles Bibliográficos
Autores principales: Widlund, Per O., Podolski, Marija, Reber, Simone, Alper, Joshua, Storch, Marko, Hyman, Anthony A., Howard, Jonathon, Drechsel, David N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3496613/
https://www.ncbi.nlm.nih.gov/pubmed/22993214
http://dx.doi.org/10.1091/mbc.E12-06-0444

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3496613/
https://www.ncbi.nlm.nih.gov/pubmed/22993214
http://dx.doi.org/10.1091/mbc.E12-06-0444

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