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Construction and Evaluation of Normalized cDNA Libraries Enriched with Full-Length Sequences for Rapid Discovery of New Genes from Sisal (Agave sisalana Perr.) Different Developmental Stages

To provide a resource of sisal-specific expressed sequence data and facilitate this powerful approach in new gene research, the preparation of normalized cDNA libraries enriched with full-length sequences is necessary. Four libraries were produced with RNA pooled from Agave sisalana multiple tissues...

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Detalles Bibliográficos
Autores principales: Zhou, Wen-Zhao, Zhang, Yan-Mei, Lu, Jun-Ying, Li, Jun-Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3497318/
https://www.ncbi.nlm.nih.gov/pubmed/23202944
http://dx.doi.org/10.3390/ijms131013150