High Affinity Humanized Antibodies without Making Hybridomas; Immunization Paired with Mammalian Cell Display and In Vitro Somatic Hypermutation

A method has been developed for the rapid generation of high-affinity humanized antibodies from immunized animals without the need to make conventional hybridomas. Rearranged IgH D(J) regions were amplified from the spleen and lymph tissue of mice immunized with the human complement protein C5, fuse...

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Detalles Bibliográficos
Autores principales: McConnell, Audrey D., Do, Minjee, Neben, Tamlyn Y., Spasojevic, Vladimir, MacLaren, Josh, Chen, Andy P., Altobell, Laurence, Macomber, John L., Berkebile, Ashley D., Horlick, Robert A., Bowers, Peter M., King, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3498135/
https://www.ncbi.nlm.nih.gov/pubmed/23166676
http://dx.doi.org/10.1371/journal.pone.0049458

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3498135/
https://www.ncbi.nlm.nih.gov/pubmed/23166676
http://dx.doi.org/10.1371/journal.pone.0049458

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