Cargando…

Biogenic amine production by the wine Lactobacillus brevis IOEB 9809 in systems that partially mimic the gastrointestinal tract stress

BACKGROUND: Ingestion of fermented foods containing high levels of biogenic amines (BA) can be deleterious to human health. Less obvious is the threat posed by BA producing organisms contained within the food which, in principle, could form BA after ingestion even if the food product itself does not...

Descripción completa

Detalles Bibliográficos
Autores principales: Russo, Pasquale, Fernández de Palencia, Pilar, Romano, Andrea, Fernández, María, Lucas, Patrick, Spano, Giuseppe, López, Paloma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3499163/
https://www.ncbi.nlm.nih.gov/pubmed/23113922
http://dx.doi.org/10.1186/1471-2180-12-247
_version_ 1782249907954909184
author Russo, Pasquale
Fernández de Palencia, Pilar
Romano, Andrea
Fernández, María
Lucas, Patrick
Spano, Giuseppe
López, Paloma
author_facet Russo, Pasquale
Fernández de Palencia, Pilar
Romano, Andrea
Fernández, María
Lucas, Patrick
Spano, Giuseppe
López, Paloma
author_sort Russo, Pasquale
collection PubMed
description BACKGROUND: Ingestion of fermented foods containing high levels of biogenic amines (BA) can be deleterious to human health. Less obvious is the threat posed by BA producing organisms contained within the food which, in principle, could form BA after ingestion even if the food product itself does not initially contain high BA levels. In this work we have investigated the production of tyramine and putrescine by Lactobacillus brevis IOEB 9809, of wine origin, under simulated gastrointestinal tract (GIT) conditions. RESULTS: An in vitro model that simulates the normal physiological conditions in the human digestive tract, as well as Caco-2 epithelial human cell lines, was used to challenge L. brevis IOEB 9809, which produced both tyramine and putrescine under all conditions tested. In the presence of BA precursors and under mild gastric stress, a correlation between enhancement of bacterial survival and a synchronous transcriptional activation of the tyramine and putrescine biosynthetic pathways was detected. High levels of both BA were observed after exposure of the bacterium to Caco-2 cells. CONCLUSIONS: L. brevis IOEB 9809 can produce tyramine and putrescine under simulated human digestive tract conditions. The results indicate that BA production may be a mechanism that increases bacterial survival under gastric stress.
format Online
Article
Text
id pubmed-3499163
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-34991632012-11-16 Biogenic amine production by the wine Lactobacillus brevis IOEB 9809 in systems that partially mimic the gastrointestinal tract stress Russo, Pasquale Fernández de Palencia, Pilar Romano, Andrea Fernández, María Lucas, Patrick Spano, Giuseppe López, Paloma BMC Microbiol Research Article BACKGROUND: Ingestion of fermented foods containing high levels of biogenic amines (BA) can be deleterious to human health. Less obvious is the threat posed by BA producing organisms contained within the food which, in principle, could form BA after ingestion even if the food product itself does not initially contain high BA levels. In this work we have investigated the production of tyramine and putrescine by Lactobacillus brevis IOEB 9809, of wine origin, under simulated gastrointestinal tract (GIT) conditions. RESULTS: An in vitro model that simulates the normal physiological conditions in the human digestive tract, as well as Caco-2 epithelial human cell lines, was used to challenge L. brevis IOEB 9809, which produced both tyramine and putrescine under all conditions tested. In the presence of BA precursors and under mild gastric stress, a correlation between enhancement of bacterial survival and a synchronous transcriptional activation of the tyramine and putrescine biosynthetic pathways was detected. High levels of both BA were observed after exposure of the bacterium to Caco-2 cells. CONCLUSIONS: L. brevis IOEB 9809 can produce tyramine and putrescine under simulated human digestive tract conditions. The results indicate that BA production may be a mechanism that increases bacterial survival under gastric stress. BioMed Central 2012-10-31 /pmc/articles/PMC3499163/ /pubmed/23113922 http://dx.doi.org/10.1186/1471-2180-12-247 Text en Copyright ©2012 Russo et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Russo, Pasquale
Fernández de Palencia, Pilar
Romano, Andrea
Fernández, María
Lucas, Patrick
Spano, Giuseppe
López, Paloma
Biogenic amine production by the wine Lactobacillus brevis IOEB 9809 in systems that partially mimic the gastrointestinal tract stress
title Biogenic amine production by the wine Lactobacillus brevis IOEB 9809 in systems that partially mimic the gastrointestinal tract stress
title_full Biogenic amine production by the wine Lactobacillus brevis IOEB 9809 in systems that partially mimic the gastrointestinal tract stress
title_fullStr Biogenic amine production by the wine Lactobacillus brevis IOEB 9809 in systems that partially mimic the gastrointestinal tract stress
title_full_unstemmed Biogenic amine production by the wine Lactobacillus brevis IOEB 9809 in systems that partially mimic the gastrointestinal tract stress
title_short Biogenic amine production by the wine Lactobacillus brevis IOEB 9809 in systems that partially mimic the gastrointestinal tract stress
title_sort biogenic amine production by the wine lactobacillus brevis ioeb 9809 in systems that partially mimic the gastrointestinal tract stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3499163/
https://www.ncbi.nlm.nih.gov/pubmed/23113922
http://dx.doi.org/10.1186/1471-2180-12-247
work_keys_str_mv AT russopasquale biogenicamineproductionbythewinelactobacillusbrevisioeb9809insystemsthatpartiallymimicthegastrointestinaltractstress
AT fernandezdepalenciapilar biogenicamineproductionbythewinelactobacillusbrevisioeb9809insystemsthatpartiallymimicthegastrointestinaltractstress
AT romanoandrea biogenicamineproductionbythewinelactobacillusbrevisioeb9809insystemsthatpartiallymimicthegastrointestinaltractstress
AT fernandezmaria biogenicamineproductionbythewinelactobacillusbrevisioeb9809insystemsthatpartiallymimicthegastrointestinaltractstress
AT lucaspatrick biogenicamineproductionbythewinelactobacillusbrevisioeb9809insystemsthatpartiallymimicthegastrointestinaltractstress
AT spanogiuseppe biogenicamineproductionbythewinelactobacillusbrevisioeb9809insystemsthatpartiallymimicthegastrointestinaltractstress
AT lopezpaloma biogenicamineproductionbythewinelactobacillusbrevisioeb9809insystemsthatpartiallymimicthegastrointestinaltractstress