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Expression Levels of the ABCG2 Multidrug Transporter in Human Erythrocytes Correspond to Pharmacologically Relevant Genetic Variations
We have developed a rapid, simple and reliable, antibody-based flow cytometry assay for the quantitative determination of membrane proteins in human erythrocytes. Our method reveals significant differences between the expression levels of the wild-type ABCG2 protein and the heterozygous Q141K polymo...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3499528/ https://www.ncbi.nlm.nih.gov/pubmed/23166586 http://dx.doi.org/10.1371/journal.pone.0048423 |
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author | Kasza, Ildikó Várady, György Andrikovics, Hajnalka Koszarska, Magdalena Tordai, Attila Scheffer, George L. Németh, Adrienn Szakács, Gergely Sarkadi, Balázs |
author_facet | Kasza, Ildikó Várady, György Andrikovics, Hajnalka Koszarska, Magdalena Tordai, Attila Scheffer, George L. Németh, Adrienn Szakács, Gergely Sarkadi, Balázs |
author_sort | Kasza, Ildikó |
collection | PubMed |
description | We have developed a rapid, simple and reliable, antibody-based flow cytometry assay for the quantitative determination of membrane proteins in human erythrocytes. Our method reveals significant differences between the expression levels of the wild-type ABCG2 protein and the heterozygous Q141K polymorphic variant. Moreover, we find that nonsense mutations on one allele result in a 50% reduction in the erythrocyte expression of this protein. Since ABCG2 polymorphisms are known to modify essential pharmacokinetic parameters, uric acid metabolism and cancer drug resistance, a direct determination of the erythrocyte membrane ABCG2 protein expression may provide valuable information for assessing these conditions or for devising drug treatments. Our findings suggest that erythrocyte membrane protein levels may reflect genotype-dependent tissue expression patterns. Extension of this methodology to other disease-related or pharmacologically important membrane proteins may yield new protein biomarkers for personalized diagnostics. |
format | Online Article Text |
id | pubmed-3499528 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34995282012-11-19 Expression Levels of the ABCG2 Multidrug Transporter in Human Erythrocytes Correspond to Pharmacologically Relevant Genetic Variations Kasza, Ildikó Várady, György Andrikovics, Hajnalka Koszarska, Magdalena Tordai, Attila Scheffer, George L. Németh, Adrienn Szakács, Gergely Sarkadi, Balázs PLoS One Research Article We have developed a rapid, simple and reliable, antibody-based flow cytometry assay for the quantitative determination of membrane proteins in human erythrocytes. Our method reveals significant differences between the expression levels of the wild-type ABCG2 protein and the heterozygous Q141K polymorphic variant. Moreover, we find that nonsense mutations on one allele result in a 50% reduction in the erythrocyte expression of this protein. Since ABCG2 polymorphisms are known to modify essential pharmacokinetic parameters, uric acid metabolism and cancer drug resistance, a direct determination of the erythrocyte membrane ABCG2 protein expression may provide valuable information for assessing these conditions or for devising drug treatments. Our findings suggest that erythrocyte membrane protein levels may reflect genotype-dependent tissue expression patterns. Extension of this methodology to other disease-related or pharmacologically important membrane proteins may yield new protein biomarkers for personalized diagnostics. Public Library of Science 2012-11-15 /pmc/articles/PMC3499528/ /pubmed/23166586 http://dx.doi.org/10.1371/journal.pone.0048423 Text en © 2012 Kasza et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kasza, Ildikó Várady, György Andrikovics, Hajnalka Koszarska, Magdalena Tordai, Attila Scheffer, George L. Németh, Adrienn Szakács, Gergely Sarkadi, Balázs Expression Levels of the ABCG2 Multidrug Transporter in Human Erythrocytes Correspond to Pharmacologically Relevant Genetic Variations |
title | Expression Levels of the ABCG2 Multidrug Transporter in Human Erythrocytes Correspond to Pharmacologically Relevant Genetic Variations |
title_full | Expression Levels of the ABCG2 Multidrug Transporter in Human Erythrocytes Correspond to Pharmacologically Relevant Genetic Variations |
title_fullStr | Expression Levels of the ABCG2 Multidrug Transporter in Human Erythrocytes Correspond to Pharmacologically Relevant Genetic Variations |
title_full_unstemmed | Expression Levels of the ABCG2 Multidrug Transporter in Human Erythrocytes Correspond to Pharmacologically Relevant Genetic Variations |
title_short | Expression Levels of the ABCG2 Multidrug Transporter in Human Erythrocytes Correspond to Pharmacologically Relevant Genetic Variations |
title_sort | expression levels of the abcg2 multidrug transporter in human erythrocytes correspond to pharmacologically relevant genetic variations |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3499528/ https://www.ncbi.nlm.nih.gov/pubmed/23166586 http://dx.doi.org/10.1371/journal.pone.0048423 |
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