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Mechanical strain promotes osteoblast ECM formation and improves its osteoinductive potential

BACKGROUND: The extracellular matrix (ECM) provides a supportive microenvironment for cells, which is suitable as a tissue engineering scaffold. Mechanical stimulus plays a significant role in the fate of osteoblast, suggesting that it regulates ECM formation. Therefore, we investigated the influenc...

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Autores principales: Guo, Yong, Zhang, Chun-qiu, Zeng, Qiang-cheng, Li, Rui-xin, Liu, Lu, Hao, Qin-xin, Shi, Cai-hong, Zhang, Xi-zheng, Yan, Yu-xian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3502495/
https://www.ncbi.nlm.nih.gov/pubmed/23098360
http://dx.doi.org/10.1186/1475-925X-11-80
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author Guo, Yong
Zhang, Chun-qiu
Zeng, Qiang-cheng
Li, Rui-xin
Liu, Lu
Hao, Qin-xin
Shi, Cai-hong
Zhang, Xi-zheng
Yan, Yu-xian
author_facet Guo, Yong
Zhang, Chun-qiu
Zeng, Qiang-cheng
Li, Rui-xin
Liu, Lu
Hao, Qin-xin
Shi, Cai-hong
Zhang, Xi-zheng
Yan, Yu-xian
author_sort Guo, Yong
collection PubMed
description BACKGROUND: The extracellular matrix (ECM) provides a supportive microenvironment for cells, which is suitable as a tissue engineering scaffold. Mechanical stimulus plays a significant role in the fate of osteoblast, suggesting that it regulates ECM formation. Therefore, we investigated the influence of mechanical stimulus on ECM formation and bioactivity. METHODS: Mouse osteoblastic MC3T3-E1 cells were cultured in cell culture dishes and stimulated with mechanical tensile strain. After removing the cells, the ECMs coated on dishes were prepared. The ECM protein and calcium were assayed and MC3T3-E1 cells were re-seeded on the ECM-coated dishes to assess osteoinductive potential of the ECM. RESULTS: The cyclic tensile strain increased collagen, bone morphogenetic protein 2 (BMP-2), BMP-4, and calcium levels in the ECM. Compared with the ECM produced by unstrained osteoblasts, those of mechanically stimulated osteoblasts promoted alkaline phosphatase activity, elevated BMP-2 and osteopontin levels and mRNA levels of runt-related transcriptional factor 2 (Runx2) and osteocalcin (OCN), and increased secreted calcium of the re-seeded MC3T3-E1 cells. CONCLUSION: Mechanical strain promoted ECM production of osteoblasts in vitro, increased BMP-2/4 levels, and improved osteoinductive potential of the ECM. This study provided a novel method to enhance bioactivity of bone ECM in vitro via mechanical strain to osteoblasts.
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spelling pubmed-35024952012-11-21 Mechanical strain promotes osteoblast ECM formation and improves its osteoinductive potential Guo, Yong Zhang, Chun-qiu Zeng, Qiang-cheng Li, Rui-xin Liu, Lu Hao, Qin-xin Shi, Cai-hong Zhang, Xi-zheng Yan, Yu-xian Biomed Eng Online Research BACKGROUND: The extracellular matrix (ECM) provides a supportive microenvironment for cells, which is suitable as a tissue engineering scaffold. Mechanical stimulus plays a significant role in the fate of osteoblast, suggesting that it regulates ECM formation. Therefore, we investigated the influence of mechanical stimulus on ECM formation and bioactivity. METHODS: Mouse osteoblastic MC3T3-E1 cells were cultured in cell culture dishes and stimulated with mechanical tensile strain. After removing the cells, the ECMs coated on dishes were prepared. The ECM protein and calcium were assayed and MC3T3-E1 cells were re-seeded on the ECM-coated dishes to assess osteoinductive potential of the ECM. RESULTS: The cyclic tensile strain increased collagen, bone morphogenetic protein 2 (BMP-2), BMP-4, and calcium levels in the ECM. Compared with the ECM produced by unstrained osteoblasts, those of mechanically stimulated osteoblasts promoted alkaline phosphatase activity, elevated BMP-2 and osteopontin levels and mRNA levels of runt-related transcriptional factor 2 (Runx2) and osteocalcin (OCN), and increased secreted calcium of the re-seeded MC3T3-E1 cells. CONCLUSION: Mechanical strain promoted ECM production of osteoblasts in vitro, increased BMP-2/4 levels, and improved osteoinductive potential of the ECM. This study provided a novel method to enhance bioactivity of bone ECM in vitro via mechanical strain to osteoblasts. BioMed Central 2012-10-25 /pmc/articles/PMC3502495/ /pubmed/23098360 http://dx.doi.org/10.1186/1475-925X-11-80 Text en Copyright ©2012 Guo et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Guo, Yong
Zhang, Chun-qiu
Zeng, Qiang-cheng
Li, Rui-xin
Liu, Lu
Hao, Qin-xin
Shi, Cai-hong
Zhang, Xi-zheng
Yan, Yu-xian
Mechanical strain promotes osteoblast ECM formation and improves its osteoinductive potential
title Mechanical strain promotes osteoblast ECM formation and improves its osteoinductive potential
title_full Mechanical strain promotes osteoblast ECM formation and improves its osteoinductive potential
title_fullStr Mechanical strain promotes osteoblast ECM formation and improves its osteoinductive potential
title_full_unstemmed Mechanical strain promotes osteoblast ECM formation and improves its osteoinductive potential
title_short Mechanical strain promotes osteoblast ECM formation and improves its osteoinductive potential
title_sort mechanical strain promotes osteoblast ecm formation and improves its osteoinductive potential
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3502495/
https://www.ncbi.nlm.nih.gov/pubmed/23098360
http://dx.doi.org/10.1186/1475-925X-11-80
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