Cargando…
Ribosomal Binding Site Switching: An Effective Strategy for High-Throughput Cloning Constructions
Direct cloning of PCR fragments by TA cloning or blunt end ligation are two simple methods which would greatly benefit high-throughput (HTP) cloning constructions if the efficiency can be improved. In this study, we have developed a ribosomal binding site (RBS) switching strategy for direct cloning...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3503710/ https://www.ncbi.nlm.nih.gov/pubmed/23185557 http://dx.doi.org/10.1371/journal.pone.0050142 |