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Ribosomal Binding Site Switching: An Effective Strategy for High-Throughput Cloning Constructions

Direct cloning of PCR fragments by TA cloning or blunt end ligation are two simple methods which would greatly benefit high-throughput (HTP) cloning constructions if the efficiency can be improved. In this study, we have developed a ribosomal binding site (RBS) switching strategy for direct cloning...

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Detalles Bibliográficos
Autores principales:	Hu, Yangbo, Feng, Lipeng, Li, Yunlong, Zhang, Yong, Lu, Pei, Rayner, Simon, Chen, Shiyun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3503710/ https://www.ncbi.nlm.nih.gov/pubmed/23185557 http://dx.doi.org/10.1371/journal.pone.0050142

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