Cargando…

Construction of a novel vector expressing the fusion suicide gene yCDglyTK and hTERT-shRNA and its antitumor effects

This study aimed to construct a novel recombinant expression vector, pcDNA3.1(-)hTERT-shRNA/yCDglyTK. Its bioactivity and antitumor effects were investigated in the SGC7901 human gastric cancer cell line. Interfering RNA (RNAi) targeting human telomerase reverse transcriptase (hTERT) was applied to...

Descripción completa

Detalles Bibliográficos
Autores principales:	LI, JIA, ZHANG, GUIYING, LIU, TING, GU, HUAN, YAN, LU, CHEN, BOLIN
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	D.A. Spandidos 2012
Materias:	Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3503886/ https://www.ncbi.nlm.nih.gov/pubmed/23181115 http://dx.doi.org/10.3892/etm.2012.613

Ejemplares similares

Effective combination gene therapy using CEACAM6-shRNA and the fusion suicide gene yCDglyTK for pancreatic carcinoma in vitro
por: LONG, HONGYU, et al.
Publicado: (2013)

Construction of a novel vector expressing Survivin-shRNA and fusion suicide gene yCDglyTK and its application in inhibiting proliferation and migration of colon cancer cells
por: Ye, Ling, et al.
Publicado: (2017)

Construction of shRNA lentiviral vector
por: Song, Hong, et al.
Publicado: (2010)

ShRNA-mediated silencing of hTERT promote apoptosis and senescence in human ovarian cancer cells
por: Su, Chen, et al.
Publicado: (2019)

Silencing of the hTERT Gene by shRNA Inhibits Colon Cancer SW480 Cell Growth In Vitro and In Vivo
por: Liu, Ai-Qun, et al.
Publicado: (2014)

Design and cloning strategies for constructing shRNA expression vectors
por: McIntyre, Glen J, et al.
Publicado: (2006)

Criteria for effective design, construction, and gene knockdown by shRNA vectors
por: Taxman, Debra J, et al.
Publicado: (2006)

Lentivirus-mediated CDglyTK gene-modified free flaps by intra-artery perfusion show targeted therapeutic efficacy in rat model of breast cancer
por: Zhang, Jianhua, et al.
Publicado: (2019)

Optimization and characterization of tRNA-shRNA expression constructs
por: Scherer, Lisa J., et al.
Publicado: (2007)

Construction and verification of the targeted uPA-shRNA lentiviral vector and evaluation of the transfection and silencing rate
por: WANG, WEI-SHAN, et al.
Publicado: (2014)

A Novel Long Non-Coding RNA in the hTERT Promoter Region Regulates hTERT Expression
por: Malhotra, Sanandan, et al.
Publicado: (2017)

The hTERT Promoter Enhances the Antitumor Activity of an Oncolytic Adenovirus under a Hypoxic Microenvironment
por: Hashimoto, Yuuri, et al.
Publicado: (2012)

Inhibition of hTERT/telomerase contributes to the antitumor activity of pristimerin in pancreatic ductal adenocarcinoma cells
por: DEEB, DORRAH, et al.
Publicado: (2015)

Construction of a lentiviral vector containing shRNA targeting ADAM17 and its role in attenuating endotoxemia in mice
por: He, Bing, et al.
Publicado: (2017)

Construction of a lentiviral vector containing shRNA targeting ADAM17 and its role in attenuating endotoxemia in mice
por: He, Bing, et al.
Publicado: (2018)

Optimized PCR Conditions and Increased shRNA Fold Representation Improve Reproducibility of Pooled shRNA Screens
por: Strezoska, Žaklina, et al.
Publicado: (2012)

An Improved Model for the hTERT Promoter Quadruplex
por: Chaires, Jonathan B., et al.
Publicado: (2014)

Lentiviral Vector Design for Multiple shRNA Expression and Durable HIV-1 Inhibition
por: Brake, Olivier ter, et al.
Publicado: (2008)

Telomere length and hTERT in mania and subsequent remission
por: Çinar, Rugül Köse
Publicado: (2017)

A novel cancer vaccine strategy with combined IL-18 and HSV-TK gene therapy driven by the hTERT promoter in a murine colorectal cancer model
por: HIGASHI, KOSUKE, et al.
Publicado: (2014)

Antithetic hTERT Regulation by Androgens in Prostate Cancer Cells: hTERT Inhibition Is Mediated by the ING1 and ING2 Tumor Suppressors
por: Bartsch, Sophie, et al.
Publicado: (2021)

hTERT DNA Methylation Analysis Identifies a Biomarker for Retinoic Acid-Induced hTERT Repression in Breast Cancer Cell Lines
por: Nguyen, Eric, et al.
Publicado: (2022)

Primary and hTERT-Transduced Mesothelioma-Associated Fibroblasts but Not Primary or hTERT-Transduced Mesothelial Cells Stimulate Growth of Human Mesothelioma Cells
por: Ries, Alexander, et al.
Publicado: (2023)

Development of a species-specific RNA polymerase I-based shRNA expression vector
por: Verca, M. S. Brenz, et al.
Publicado: (2007)

Efficient shRNA delivery into B and T lymphoma cells using lentiviral vector-mediated transfer
por: Anastasov, Nataša, et al.
Publicado: (2008)

A Simple and Robust Vector-Based shRNA Expression System Used for RNA Interference
por: Wang, Xue-jun, et al.
Publicado: (2013)

Regulation and clinical potential of telomerase reverse transcriptase (TERT/hTERT) in breast cancer
por: Yang, Ruozhu, et al.
Publicado: (2023)

shRNA target prediction informed by comprehensive enquiry (SPICE): a supporting system for high-throughput screening of shRNA library
por: Kamatuka, Kenta, et al.
Publicado: (2016)

Dual roles of c-Myc in the regulation of hTERT gene
por: Zhao, Yuanjun, et al.
Publicado: (2014)

The intrabody targeting of hTERT attenuates the immortality of cancer cells
por: Zhu, Xiangying, et al.
Publicado: (2009)

Exploring hTERT promoter methylation in cutaneous T‐cell lymphomas
por: Chebly, Alain, et al.
Publicado: (2021)

Spotlight on hTERT Complex Regulation in Cutaneous T-Cell Lymphomas
por: Ropio, Joana, et al.
Publicado: (2023)

Cassette deletion in multiple shRNA lentiviral vectors for HIV-1 and its impact on treatment success
por: Mcintyre, Glen J, et al.
Publicado: (2009)

A multi-shRNA vector enhances the silencing efficiency of exogenous and endogenous genes in human cells
por: Weng, Yanjie, et al.
Publicado: (2017)

hTERT mRNA dendritic cell vaccination: complete response in a pancreatic cancer patient associated with response against several hTERT epitopes
por: Suso, Else M. Inderberg, et al.
Publicado: (2011)

Widespread CD4+ T-cell reactivity to novel hTERT epitopes following vaccination of cancer patients with a single hTERT peptide GV1001
por: Inderberg-Suso, Else-Marit, et al.
Publicado: (2012)

RNAi Codex: a portal/database for short-hairpin RNA (shRNA) gene-silencing constructs
por: Olson, A., et al.
Publicado: (2006)

Image-aided Suicide Gene Therapy Utilizing Multifunctional hTERT-targeting Adenovirus for Clinical Translation in Hepatocellular Carcinoma
por: Kim, Yun-Hee, et al.
Publicado: (2016)

Construction of a human hTERT RPE-1 cell line with inducible Cre for editing of endogenous genes
por: Hindul, Naushin L., et al.
Publicado: (2022)

A simple approach for multi-targeted shRNA-mediated inducible knockdowns using Sleeping Beauty vectors
por: Fink, Severin, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_6c63tc4smp938pk6ie4op83g9a