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Thermal, Chemical and pH Induced Denaturation of a Multimeric β-Galactosidase Reveals Multiple Unfolding Pathways

BACKGROUND: In this case study, we analysed the properties of unfolded states and pathways leading to complete denaturation of a multimeric chick pea β-galactosidase (CpGAL), as obtained from treatment with guanidium hydrochloride, urea, elevated temperature and extreme pH. METHODOLOGY/PRINCIPAL FIN...

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Detalles Bibliográficos
Autores principales: Kishore, Devesh, Kundu, Suman, Kayastha, Arvind M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3503960/
https://www.ncbi.nlm.nih.gov/pubmed/23185611
http://dx.doi.org/10.1371/journal.pone.0050380