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Functional Consequences of Sulfhydryl Modification of the γ-Aminobutyric Acid Transporter 1 at a Single Solvent-Exposed Cysteine Residue

The aims of this study were to optimize the experimental conditions for labeling extracellularly oriented, solvent-exposed cysteine residues of γ-aminobutyric acid transporter 1 (GAT1) with the membrane-impermeant sulfhydryl reagent [2-(trimethylammonium)ethyl]methanethiosulfonate (MTSET) and to cha...

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Detalles Bibliográficos
Autores principales: Omoto, Jaison J., Maestas, Matthew J., Rahnama-Vaghef, Ali, Choi, Ye E., Salto, Gerardo, Sanchez, Rachel V., Anderson, Cynthia M., Eskandari, Sepehr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3505503/
https://www.ncbi.nlm.nih.gov/pubmed/22918627
http://dx.doi.org/10.1007/s00232-012-9492-9