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Functional Consequences of Sulfhydryl Modification of the γ-Aminobutyric Acid Transporter 1 at a Single Solvent-Exposed Cysteine Residue
The aims of this study were to optimize the experimental conditions for labeling extracellularly oriented, solvent-exposed cysteine residues of γ-aminobutyric acid transporter 1 (GAT1) with the membrane-impermeant sulfhydryl reagent [2-(trimethylammonium)ethyl]methanethiosulfonate (MTSET) and to cha...
Autores principales: | Omoto, Jaison J., Maestas, Matthew J., Rahnama-Vaghef, Ali, Choi, Ye E., Salto, Gerardo, Sanchez, Rachel V., Anderson, Cynthia M., Eskandari, Sepehr |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3505503/ https://www.ncbi.nlm.nih.gov/pubmed/22918627 http://dx.doi.org/10.1007/s00232-012-9492-9 |
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