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Culture parameters for stable expansion, genetic modification and germline transmission of rat pluripotent stem cells

The ability of cultured pluripotent cells to contribute to the germline of chimaeric animals is essential to their utility for genetic manipulation. In the three years since rat embryonic stem (ES) cells were first reported the anticipated proliferation of genetically modified rat models from this n...

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Detalles Bibliográficos
Autores principales: Blair, Kathryn, Leitch, Harry G., Mansfield, William, Dumeau, Charles-Étienne, Humphreys, Peter, Smith, Austin G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507162/
https://www.ncbi.nlm.nih.gov/pubmed/23213369
http://dx.doi.org/10.1242/bio.2011029