Cargando…

Dual pathway spindle assembly increases both the speed and the fidelity of mitosis

Roughly half of all animal somatic cell spindles assemble by the classical prophase pathway, in which the centrosomes separate ahead of nuclear envelope breakdown (NEBD). The remainder assemble by the prometaphase pathway, in which the centrosomes separate following NEBD. Why cells use dual pathway...

Descripción completa

Detalles Bibliográficos
Autores principales: Kaseda, Kuniyoshi, McAinsh, Andrew D., Cross, Robert A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507165/
https://www.ncbi.nlm.nih.gov/pubmed/23213363
http://dx.doi.org/10.1242/bio.2011012
_version_ 1782251029510750208
author Kaseda, Kuniyoshi
McAinsh, Andrew D.
Cross, Robert A.
author_facet Kaseda, Kuniyoshi
McAinsh, Andrew D.
Cross, Robert A.
author_sort Kaseda, Kuniyoshi
collection PubMed
description Roughly half of all animal somatic cell spindles assemble by the classical prophase pathway, in which the centrosomes separate ahead of nuclear envelope breakdown (NEBD). The remainder assemble by the prometaphase pathway, in which the centrosomes separate following NEBD. Why cells use dual pathway spindle assembly is unclear. Here, by examining the timing of NEBD relative to the onset of Eg5-mEGFP loading to centrosomes, we show that a time window of 9.2 ± 2.9 min is available for Eg5-driven prophase centrosome separation ahead of NEBD, and that those cells that succeed in separating their centrosomes within this window subsequently show >3-fold fewer chromosome segregation errors and a somewhat faster mitosis. A longer time window would allow more cells to complete prophase centrosome separation and further reduce segregation errors, but at the expense of a slower mitosis. Our data reveal dual pathway mitosis in a new light, as a substantive strategy that increases both the speed and the fidelity of mitosis.
format Online
Article
Text
id pubmed-3507165
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher The Company of Biologists
record_format MEDLINE/PubMed
spelling pubmed-35071652012-12-04 Dual pathway spindle assembly increases both the speed and the fidelity of mitosis Kaseda, Kuniyoshi McAinsh, Andrew D. Cross, Robert A. Biol Open Research Article Roughly half of all animal somatic cell spindles assemble by the classical prophase pathway, in which the centrosomes separate ahead of nuclear envelope breakdown (NEBD). The remainder assemble by the prometaphase pathway, in which the centrosomes separate following NEBD. Why cells use dual pathway spindle assembly is unclear. Here, by examining the timing of NEBD relative to the onset of Eg5-mEGFP loading to centrosomes, we show that a time window of 9.2 ± 2.9 min is available for Eg5-driven prophase centrosome separation ahead of NEBD, and that those cells that succeed in separating their centrosomes within this window subsequently show >3-fold fewer chromosome segregation errors and a somewhat faster mitosis. A longer time window would allow more cells to complete prophase centrosome separation and further reduce segregation errors, but at the expense of a slower mitosis. Our data reveal dual pathway mitosis in a new light, as a substantive strategy that increases both the speed and the fidelity of mitosis. The Company of Biologists 2011-10-24 /pmc/articles/PMC3507165/ /pubmed/23213363 http://dx.doi.org/10.1242/bio.2011012 Text en © 2011. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by-nc-sa/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Article
Kaseda, Kuniyoshi
McAinsh, Andrew D.
Cross, Robert A.
Dual pathway spindle assembly increases both the speed and the fidelity of mitosis
title Dual pathway spindle assembly increases both the speed and the fidelity of mitosis
title_full Dual pathway spindle assembly increases both the speed and the fidelity of mitosis
title_fullStr Dual pathway spindle assembly increases both the speed and the fidelity of mitosis
title_full_unstemmed Dual pathway spindle assembly increases both the speed and the fidelity of mitosis
title_short Dual pathway spindle assembly increases both the speed and the fidelity of mitosis
title_sort dual pathway spindle assembly increases both the speed and the fidelity of mitosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507165/
https://www.ncbi.nlm.nih.gov/pubmed/23213363
http://dx.doi.org/10.1242/bio.2011012
work_keys_str_mv AT kasedakuniyoshi dualpathwayspindleassemblyincreasesboththespeedandthefidelityofmitosis
AT mcainshandrewd dualpathwayspindleassemblyincreasesboththespeedandthefidelityofmitosis
AT crossroberta dualpathwayspindleassemblyincreasesboththespeedandthefidelityofmitosis