A simple protocol for the subcellular fractionation of skeletal muscle cells and tissue

BACKGROUND: We describe a method for subcellular fractionation of mouse skeletal muscle, myoblast and myotubes to obtain relatively pure fractions of nuclear, cytosolic and mitochondrial compartments. Fractionation allows the analysis of a protein of interest (or other cellular component) based on i...

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Detalles Bibliográficos
Autores principales: Dimauro, Ivan, Pearson, Timothy, Caporossi, Daniela, Jackson, Malcolm J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3508861/
https://www.ncbi.nlm.nih.gov/pubmed/22994964
http://dx.doi.org/10.1186/1756-0500-5-513

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3508861/
https://www.ncbi.nlm.nih.gov/pubmed/22994964
http://dx.doi.org/10.1186/1756-0500-5-513

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