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A simple protocol for the subcellular fractionation of skeletal muscle cells and tissue

BACKGROUND: We describe a method for subcellular fractionation of mouse skeletal muscle, myoblast and myotubes to obtain relatively pure fractions of nuclear, cytosolic and mitochondrial compartments. Fractionation allows the analysis of a protein of interest (or other cellular component) based on i...

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Detalles Bibliográficos
Autores principales:	Dimauro, Ivan, Pearson, Timothy, Caporossi, Daniela, Jackson, Malcolm J
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2012
Materias:	Technical Note
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3508861/ https://www.ncbi.nlm.nih.gov/pubmed/22994964 http://dx.doi.org/10.1186/1756-0500-5-513

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