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Monitoring Biosensor Activity in Living Cells with Fluorescence Lifetime Imaging Microscopy

Live-cell microscopy is now routinely used to monitor the activities of the genetically encoded biosensor proteins that are designed to directly measure specific cell signaling events inside cells, tissues, or organisms. Most fluorescent biosensor proteins rely on Förster resonance energy transfer (...

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Detalles Bibliográficos
Autores principales: Hum, Julia M., Siegel, Amanda P., Pavalko, Fredrick M., Day, Richard N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509586/
https://www.ncbi.nlm.nih.gov/pubmed/23203070
http://dx.doi.org/10.3390/ijms131114385