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1-Anilino-8-Naphthalene Sulfonate (ANS) Is Not a Desirable Probe for Determining the Molten Globule State of Chymopapain

The molten globule (MG) state of proteins is widely detected through binding with 1-anilino-8-naphthalene sulphonate (ANS), a fluorescent dye. This strategy is based upon the assumption that when in molten globule state, the exposed hydrophobic clusters of protein are readily bound by the nonpolar a...

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Detalles Bibliográficos
Autores principales: Qadeer, Atiyatul, Rabbani, Gulam, Zaidi, Nida, Ahmad, Ejaz, Khan, Javed M., Khan, Rizwan H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3510187/
https://www.ncbi.nlm.nih.gov/pubmed/23209794
http://dx.doi.org/10.1371/journal.pone.0050633