Cargando…

Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction

PURPOSE: Methods for quick and reliable detection of Streptococcus pneumoniae are needed for the diagnosis of pneumococcal disease and vaccine studies. This study aimed to show that sequential multiplex polymerase chain reaction (PCR) is more efficient than conventional culture in achieving S. pneum...

Descripción completa

Detalles Bibliográficos
Autores principales: Ahn, Jong Gyun, Choi, Seong Yeol, Kim, Dong Soo, Kim, Ki Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Pediatric Society 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3510272/
https://www.ncbi.nlm.nih.gov/pubmed/23227062
http://dx.doi.org/10.3345/kjp.2012.55.11.424
_version_ 1782251447991140352
author Ahn, Jong Gyun
Choi, Seong Yeol
Kim, Dong Soo
Kim, Ki Hwan
author_facet Ahn, Jong Gyun
Choi, Seong Yeol
Kim, Dong Soo
Kim, Ki Hwan
author_sort Ahn, Jong Gyun
collection PubMed
description PURPOSE: Methods for quick and reliable detection of Streptococcus pneumoniae are needed for the diagnosis of pneumococcal disease and vaccine studies. This study aimed to show that sequential multiplex polymerase chain reaction (PCR) is more efficient than conventional culture in achieving S. pneumoniae-positive results. METHODS: Nasopharyngeal (NP) secretions were obtained from 842 pediatric patients admitted with lower respiratory infections at Severance Children's Hospital in Korea between March 2009 and June 2010. For identification and serotype determination of pneumococci from the NP secretions, the secretions were evaluated via multiplex PCR technique with 35 serotype-specific primers arranged in 8 multiplex PCR sets and conventional bacteriological culture technique. RESULTS: Among the results for 793 samples that underwent both bacterial culture and PCR analysis for pneumococcal detection, 153 (19.3%) results obtained by PCR and 81 (10.2%) results obtained by conventional culture technique were positive for S. pneumoniae. The predominant serotypes observed, in order of decreasing frequency, were 19A (23%), 6A/B (16%), 19F (11%), 15B/C (5%), 15A (5%), and 11A (4%); further, 26% of the isolates were non-typeable. CONCLUSION: As opposed to conventional bacteriological tests, PCR analysis can accurately and rapidly identify pneumococcal serotypes.
format Online
Article
Text
id pubmed-3510272
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher The Korean Pediatric Society
record_format MEDLINE/PubMed
spelling pubmed-35102722012-12-07 Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction Ahn, Jong Gyun Choi, Seong Yeol Kim, Dong Soo Kim, Ki Hwan Korean J Pediatr Original Article PURPOSE: Methods for quick and reliable detection of Streptococcus pneumoniae are needed for the diagnosis of pneumococcal disease and vaccine studies. This study aimed to show that sequential multiplex polymerase chain reaction (PCR) is more efficient than conventional culture in achieving S. pneumoniae-positive results. METHODS: Nasopharyngeal (NP) secretions were obtained from 842 pediatric patients admitted with lower respiratory infections at Severance Children's Hospital in Korea between March 2009 and June 2010. For identification and serotype determination of pneumococci from the NP secretions, the secretions were evaluated via multiplex PCR technique with 35 serotype-specific primers arranged in 8 multiplex PCR sets and conventional bacteriological culture technique. RESULTS: Among the results for 793 samples that underwent both bacterial culture and PCR analysis for pneumococcal detection, 153 (19.3%) results obtained by PCR and 81 (10.2%) results obtained by conventional culture technique were positive for S. pneumoniae. The predominant serotypes observed, in order of decreasing frequency, were 19A (23%), 6A/B (16%), 19F (11%), 15B/C (5%), 15A (5%), and 11A (4%); further, 26% of the isolates were non-typeable. CONCLUSION: As opposed to conventional bacteriological tests, PCR analysis can accurately and rapidly identify pneumococcal serotypes. The Korean Pediatric Society 2012-11 2012-11-23 /pmc/articles/PMC3510272/ /pubmed/23227062 http://dx.doi.org/10.3345/kjp.2012.55.11.424 Text en Copyright © 2012 by The Korean Pediatric Society http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ahn, Jong Gyun
Choi, Seong Yeol
Kim, Dong Soo
Kim, Ki Hwan
Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction
title Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction
title_full Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction
title_fullStr Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction
title_full_unstemmed Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction
title_short Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction
title_sort enhanced detection and serotyping of streptococcus pneumoniae using multiplex polymerase chain reaction
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3510272/
https://www.ncbi.nlm.nih.gov/pubmed/23227062
http://dx.doi.org/10.3345/kjp.2012.55.11.424
work_keys_str_mv AT ahnjonggyun enhanceddetectionandserotypingofstreptococcuspneumoniaeusingmultiplexpolymerasechainreaction
AT choiseongyeol enhanceddetectionandserotypingofstreptococcuspneumoniaeusingmultiplexpolymerasechainreaction
AT kimdongsoo enhanceddetectionandserotypingofstreptococcuspneumoniaeusingmultiplexpolymerasechainreaction
AT kimkihwan enhanceddetectionandserotypingofstreptococcuspneumoniaeusingmultiplexpolymerasechainreaction