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Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction
PURPOSE: Methods for quick and reliable detection of Streptococcus pneumoniae are needed for the diagnosis of pneumococcal disease and vaccine studies. This study aimed to show that sequential multiplex polymerase chain reaction (PCR) is more efficient than conventional culture in achieving S. pneum...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Pediatric Society
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3510272/ https://www.ncbi.nlm.nih.gov/pubmed/23227062 http://dx.doi.org/10.3345/kjp.2012.55.11.424 |
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author | Ahn, Jong Gyun Choi, Seong Yeol Kim, Dong Soo Kim, Ki Hwan |
author_facet | Ahn, Jong Gyun Choi, Seong Yeol Kim, Dong Soo Kim, Ki Hwan |
author_sort | Ahn, Jong Gyun |
collection | PubMed |
description | PURPOSE: Methods for quick and reliable detection of Streptococcus pneumoniae are needed for the diagnosis of pneumococcal disease and vaccine studies. This study aimed to show that sequential multiplex polymerase chain reaction (PCR) is more efficient than conventional culture in achieving S. pneumoniae-positive results. METHODS: Nasopharyngeal (NP) secretions were obtained from 842 pediatric patients admitted with lower respiratory infections at Severance Children's Hospital in Korea between March 2009 and June 2010. For identification and serotype determination of pneumococci from the NP secretions, the secretions were evaluated via multiplex PCR technique with 35 serotype-specific primers arranged in 8 multiplex PCR sets and conventional bacteriological culture technique. RESULTS: Among the results for 793 samples that underwent both bacterial culture and PCR analysis for pneumococcal detection, 153 (19.3%) results obtained by PCR and 81 (10.2%) results obtained by conventional culture technique were positive for S. pneumoniae. The predominant serotypes observed, in order of decreasing frequency, were 19A (23%), 6A/B (16%), 19F (11%), 15B/C (5%), 15A (5%), and 11A (4%); further, 26% of the isolates were non-typeable. CONCLUSION: As opposed to conventional bacteriological tests, PCR analysis can accurately and rapidly identify pneumococcal serotypes. |
format | Online Article Text |
id | pubmed-3510272 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Korean Pediatric Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-35102722012-12-07 Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction Ahn, Jong Gyun Choi, Seong Yeol Kim, Dong Soo Kim, Ki Hwan Korean J Pediatr Original Article PURPOSE: Methods for quick and reliable detection of Streptococcus pneumoniae are needed for the diagnosis of pneumococcal disease and vaccine studies. This study aimed to show that sequential multiplex polymerase chain reaction (PCR) is more efficient than conventional culture in achieving S. pneumoniae-positive results. METHODS: Nasopharyngeal (NP) secretions were obtained from 842 pediatric patients admitted with lower respiratory infections at Severance Children's Hospital in Korea between March 2009 and June 2010. For identification and serotype determination of pneumococci from the NP secretions, the secretions were evaluated via multiplex PCR technique with 35 serotype-specific primers arranged in 8 multiplex PCR sets and conventional bacteriological culture technique. RESULTS: Among the results for 793 samples that underwent both bacterial culture and PCR analysis for pneumococcal detection, 153 (19.3%) results obtained by PCR and 81 (10.2%) results obtained by conventional culture technique were positive for S. pneumoniae. The predominant serotypes observed, in order of decreasing frequency, were 19A (23%), 6A/B (16%), 19F (11%), 15B/C (5%), 15A (5%), and 11A (4%); further, 26% of the isolates were non-typeable. CONCLUSION: As opposed to conventional bacteriological tests, PCR analysis can accurately and rapidly identify pneumococcal serotypes. The Korean Pediatric Society 2012-11 2012-11-23 /pmc/articles/PMC3510272/ /pubmed/23227062 http://dx.doi.org/10.3345/kjp.2012.55.11.424 Text en Copyright © 2012 by The Korean Pediatric Society http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Ahn, Jong Gyun Choi, Seong Yeol Kim, Dong Soo Kim, Ki Hwan Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction |
title | Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction |
title_full | Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction |
title_fullStr | Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction |
title_full_unstemmed | Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction |
title_short | Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction |
title_sort | enhanced detection and serotyping of streptococcus pneumoniae using multiplex polymerase chain reaction |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3510272/ https://www.ncbi.nlm.nih.gov/pubmed/23227062 http://dx.doi.org/10.3345/kjp.2012.55.11.424 |
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