Transphosphorylation of E. coli Proteins during Production of Recombinant Protein Kinases Provides a Robust System to Characterize Kinase Specificity

Protein kinase specificity is of fundamental importance to pathway regulation and signal transduction. Here, we report a convenient system to monitor the activity and specificity of recombinant protein kinases expressed in E. coli. We apply this to the study of the cytoplasmic domain of the plant re...

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Detalles Bibliográficos
Autores principales: Wu, Xia, Oh, Man-Ho, Kim, Hyoung Seok, Schwartz, Daniel, Imai, Brian S., Yau, Peter M., Clouse, Steven D., Huber, Steven C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2012
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3510383/
https://www.ncbi.nlm.nih.gov/pubmed/23226150
http://dx.doi.org/10.3389/fpls.2012.00262

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3510383/
https://www.ncbi.nlm.nih.gov/pubmed/23226150
http://dx.doi.org/10.3389/fpls.2012.00262

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