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Exploiting the Intron-splicing Mechanism of Insect Cells to Produce Viral Vectors Harboring Toxic Genes for Suicide Gene Therapy
Two mammalian introns, the human growth hormone intron and the Simian virus 40 large T antigen intron, were inserted into the coding sequences of diphtheria toxin fragment A (DT-A) and barnase (Bar), respectively, to disrupt their open-reading frames (ORFs). Expression of these two toxic proteins we...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group
2012
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3511675/ https://www.ncbi.nlm.nih.gov/pubmed/23187456 http://dx.doi.org/10.1038/mtna.2012.48 |